Enzymatic synthesis of a sulfate-containing nucleotide, inosine-5'-phosphosulfate, in several marine macroalgae

N. Kanno, M. Sato, Y. Sato

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    3 Citations (Scopus)

    Abstract

    In a study on the sulfate-activating system of marine macroalgae, an interesting sulfate-containing nucleotide was found as the enzymatic reaction product, and was isolated by DEAE-Toyo Pearl column chromatography. This nucleotide showed an ultraviolet spectrum similar to that of IMP, and had an acid-labile phosphate-sulfate anhydride bond at the 5' position. It was identified as inosine-5'-phosphosulfate by comparison with a synthetic specimen. It was speculated that inosine-5'-phosphosulfate was formed from the active sulfate, adenosine-5'-phosphosulfate, by the enzymatic deamination.

    Original languageEnglish
    Pages (from-to)519-528
    Number of pages10
    JournalBiochemistry International
    Volume21
    Issue number3
    Publication statusPublished - 1990 Aug 23

    ASJC Scopus subject areas

    • Biochemistry

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