TY - JOUR
T1 - Enzymatic ring-opening polyaddition for chitin synthesis
T2 - A cationic mechanism in basic solution?
AU - Kiyosada, Toshitsugu
AU - Shoda, Shin Ichiro
AU - Kobayashi, Shiro
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1998/7
Y1 - 1998/7
N2 - The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.
AB - The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.
UR - http://www.scopus.com/inward/record.url?scp=0032354423&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032354423&partnerID=8YFLogxK
U2 - 10.1002/masy.19981320138
DO - 10.1002/masy.19981320138
M3 - Article
AN - SCOPUS:0032354423
VL - 132
SP - 415
EP - 420
JO - Macromolecular Symposia
JF - Macromolecular Symposia
SN - 1022-1360
ER -