Enzymatic ring-opening polyaddition for chitin synthesis: A cationic mechanism in basic solution?

Toshitsugu Kiyosada; Shin Ichiro Shoda; Shiro Kobayashi

doi:10.1002/masy.19981320138

Enzymatic ring-opening polyaddition for chitin synthesis: A cationic mechanism in basic solution?

Toshitsugu Kiyosada, Shin Ichiro Shoda, Shiro Kobayashi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS ¹³C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.

Original language	English
Pages (from-to)	415-420
Number of pages	6
Journal	Macromolecular Symposia
Volume	132
DOIs	https://doi.org/10.1002/masy.19981320138
Publication status	Published - 1998 Jul
Externally published	Yes

ASJC Scopus subject areas

Condensed Matter Physics
Organic Chemistry
Polymers and Plastics
Materials Chemistry

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10.1002/masy.19981320138

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title = "Enzymatic ring-opening polyaddition for chitin synthesis: A cationic mechanism in basic solution?",

abstract = "The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.",

author = "Toshitsugu Kiyosada and Shoda, {Shin Ichiro} and Shiro Kobayashi",

year = "1998",

month = jul,

doi = "10.1002/masy.19981320138",

language = "English",

volume = "132",

pages = "415--420",

journal = "Macromolecular Symposia",

issn = "1022-1360",

publisher = "Wiley-VCH Verlag",

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TY - JOUR

T1 - Enzymatic ring-opening polyaddition for chitin synthesis

T2 - A cationic mechanism in basic solution?

AU - Kiyosada, Toshitsugu

AU - Shoda, Shin Ichiro

AU - Kobayashi, Shiro

PY - 1998/7

Y1 - 1998/7

N2 - The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.

AB - The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.

UR - http://www.scopus.com/inward/record.url?scp=0032354423&partnerID=8YFLogxK

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DO - 10.1002/masy.19981320138

M3 - Article

AN - SCOPUS:0032354423

VL - 132

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EP - 420

JO - Macromolecular Symposia

JF - Macromolecular Symposia

SN - 1022-1360

ER -

Enzymatic ring-opening polyaddition for chitin synthesis: A cationic mechanism in basic solution?

Abstract

ASJC Scopus subject areas

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