Enzymatic ring-opening polyaddition for chitin synthesis: A cationic mechanism in basic solution?

Toshitsugu Kiyosada, Shin Ichiro Shoda, Shiro Kobayashi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The in vitro synthesis of chitin via a nonbiosynthetic path has been achieved for the first time by enzymatic ring-opening polyaddition of a chitobiose oxazoline monomer (C2O). Chitinase, a hydrolysis enzyme of chitin, recognized and polymerized the monomer regio- and stereoselectively. The structure of artificial chitin was confirmed by comparison with an authentic natural chitin sample with the use of CP/MAS 13C NMR and IR spectroscopy. X-ray diffraction as well as NMR analysis showed its crystal structure of α-chitin. Characteristic features of the present polymerization are described.

Original languageEnglish
Pages (from-to)415-420
Number of pages6
JournalMacromolecular Symposia
Volume132
DOIs
Publication statusPublished - 1998 Jul

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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