Enzymatic photochemical sensing using luciferase-immobilized polymer nanoparticles covered with artificial cell membrane

Tomohiro Konno, Tomomi Ito, Madoka Takai, Kazuhiko Ishihara

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We prepared phospholipid polymer nanoparticles immobilized with luciferase, and the nanoparticles were applied as photochemical sensing nanoparticles. An amphiphilic water-soluble polymer having a phosphorylcholine group was used as an emulsifier and a surface modifier to prepare the nanoparticles. The polymer was composed of three kinds of monomer units, that is, 2-methacryloyloxyethyl phosphorylcholine (MPC) as a hydrophilic and bioinert unit, n-butyl methacrylate as a hydrophobic unit and p-nitrophenyl ester having methacrylate as an enzyme-immobilizing unit. The p-nitrophenyl ester groups to immobilize the proteins were located on the surface of the nanoparticles. Luciferase was immobilized by the reaction between the p-nitrophenyl ester groups and the amino group. The enzymatic reaction on the nanoparticles was followed using a microdialysis system with an optical fiber having a 800 μm diameter in the probe. The nanoparticles conjugated with luciferase reacted with ATP, luciferin and oxygen. It is concluded that the nanoparticles are a promising tool for a photochemical sensing microdiagnostic system.

Original languageEnglish
Pages (from-to)1347-1357
Number of pages11
JournalJournal of Biomaterials Science, Polymer Edition
Volume17
Issue number12
DOIs
Publication statusPublished - 2006 Dec
Externally publishedYes

Keywords

  • 2-Methacryloyloxyethyl phosphorylcholine polymer
  • Chemical luminescence
  • Enzymatic reaction
  • Nanoparticles
  • Photosensing system

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

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