Enzymatic oxidative polymerization of 4-chloroguaiacol by laccase

Takaaki Tanaka, Mihoko Yamamoto, Masafumi Takahashi, Tomoyuki Fujii, Masayuki Taniguchi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The kinetics of enzymatic oxidative polymerization of 4-chloroguaiacol by laccase was studied. 4-Chloroguaiacol, a model compound of the chloroguaiacols formed in the bleaching process in the pulp industry, was oxidized by laccase from Trametes sp. Hal to eliminate chloroguaiacols from pulp wastewater by polymerization and precipitation. On the basis of the reaction rate obtained from the decrease in the 4-chloroguaiacol concentration, the optimum pH of the reaction was 4-5 and the apparent Michaelis constant Km for the reaction was more than 5 mmol dm-3. However, the value of Km obtained from the dependence of oxygen consumption rate on the concentration of 4-chloroguaiacol was 0.092 mmol dm-3. The reaction rate was expressed as the sum of the enzymatic oxidation rate of 4-chloroguaiacol and the polymerization rate of the radical formed in the enzymatic oxidation. The kinetics of the enzymatic oxidative polymerization shown in this study will be useful to understand those of the enzymatic oxidation of other phenolic compounds.

Original languageEnglish
Pages (from-to)1101-1106
Number of pages6
JournalJOURNAL of CHEMICAL ENGINEERING of JAPAN
Volume36
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1
Externally publishedYes

Keywords

  • Chloroguaiacol
  • Enzymatic oxidative polymerization
  • Kinetics
  • Laccase
  • Wastewater treatment

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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