Abstract
Modified nucleotides are incorporated by enzymatic reactions into a DNA oligonulceotide to provide additional functionalities, which has shown potential applications in a wide variety of fields. A large number of modified nucleotide triphosphates have been incorporated to DNA or RNA by polymerases. We have previously described that the sulfide derivatives (1) of 2-amino-6-vinyl purine nucleoside (2, AVP) are activated within duplex to form cross-link toward cytidine selectively at the target site. In this paper, we describe the preparation of the triphosphate derivative of 2'-deoxyribosyl-2-amino-6-(2-methylthioethyl) purine nucleoside (5, dAVP(S Me)-TP), and its enzymatic incorporation to DNA.
Original language | English |
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Pages (from-to) | 357-358 |
Number of pages | 2 |
Journal | Nucleic acids symposium series (2004) |
Issue number | 51 |
DOIs | |
Publication status | Published - 2007 |
ASJC Scopus subject areas
- Medicine(all)