Enzymatic hydrolysis of cellulose recovered from ionic liquid-salt aqueous two-phase system

Kazuhiko Tanimura, Keishi Suga, Yukihiro Okamoto, Hiroshi Umakoshi

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Regenerated cellulose can be prepared by treatment with an ionic liquid (IL) and an anti-solvent such as water, which significantly enhances the enzymatic hydrolysis in comparison to crystalline cellulose. The IL-aqueous two-phase system (IL-ATPS) is consisted of IL-condensed top phase and salt-condensed bottom phase, which could be suitable to produce regenerated cellulose with smaller amount of IL. Using IL-ATPS with different pH, the enzymatic saccharification efficiency of crystalline cellulose was determined. The use of 1-allyl-3-methylimidazolium chloride resulted in relatively higher yield of glucose production as compared to 1-butyl-3-methylimidazolium chloride. The IL-ATPS showing optimal pH for cellulase was prepared with mixed salt (NaH2PO4/Na2HPO4 = 5/1 (wt/wt)), which provide a regenerated cellulose with the pH range of 4.8–4.9 in enzymatic reaction mixture. Using such regenerated cellulose as feed of saccharification, the final yield of glucose was about 70%.

Original languageEnglish
Pages (from-to)624-631
Number of pages8
JournalJournal of Bioscience and Bioengineering
Issue number5
Publication statusPublished - 2020 May
Externally publishedYes


  • Cellulase
  • Cellulose
  • Ionic liquid: aqueous two-phase system

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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