TY - JOUR
T1 - Enzymatic hydrolysis of cellulose recovered from ionic liquid-salt aqueous two-phase system
AU - Tanimura, Kazuhiko
AU - Suga, Keishi
AU - Okamoto, Yukihiro
AU - Umakoshi, Hiroshi
N1 - Funding Information:
This research was supported by the Japan Society for the Promotion of Science (JSPS) Grant-in-Aid for Scientific Research A ( 26249116 ) and the JSPS Grant-in-Aid for Challenging Exploratory Research ( T15K142040 ). Appendix A
Publisher Copyright:
© 2020 The Society for Biotechnology, Japan
PY - 2020/5
Y1 - 2020/5
N2 - Regenerated cellulose can be prepared by treatment with an ionic liquid (IL) and an anti-solvent such as water, which significantly enhances the enzymatic hydrolysis in comparison to crystalline cellulose. The IL-aqueous two-phase system (IL-ATPS) is consisted of IL-condensed top phase and salt-condensed bottom phase, which could be suitable to produce regenerated cellulose with smaller amount of IL. Using IL-ATPS with different pH, the enzymatic saccharification efficiency of crystalline cellulose was determined. The use of 1-allyl-3-methylimidazolium chloride resulted in relatively higher yield of glucose production as compared to 1-butyl-3-methylimidazolium chloride. The IL-ATPS showing optimal pH for cellulase was prepared with mixed salt (NaH2PO4/Na2HPO4 = 5/1 (wt/wt)), which provide a regenerated cellulose with the pH range of 4.8–4.9 in enzymatic reaction mixture. Using such regenerated cellulose as feed of saccharification, the final yield of glucose was about 70%.
AB - Regenerated cellulose can be prepared by treatment with an ionic liquid (IL) and an anti-solvent such as water, which significantly enhances the enzymatic hydrolysis in comparison to crystalline cellulose. The IL-aqueous two-phase system (IL-ATPS) is consisted of IL-condensed top phase and salt-condensed bottom phase, which could be suitable to produce regenerated cellulose with smaller amount of IL. Using IL-ATPS with different pH, the enzymatic saccharification efficiency of crystalline cellulose was determined. The use of 1-allyl-3-methylimidazolium chloride resulted in relatively higher yield of glucose production as compared to 1-butyl-3-methylimidazolium chloride. The IL-ATPS showing optimal pH for cellulase was prepared with mixed salt (NaH2PO4/Na2HPO4 = 5/1 (wt/wt)), which provide a regenerated cellulose with the pH range of 4.8–4.9 in enzymatic reaction mixture. Using such regenerated cellulose as feed of saccharification, the final yield of glucose was about 70%.
KW - Cellulase
KW - Cellulose
KW - Ionic liquid: aqueous two-phase system
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U2 - 10.1016/j.jbiosc.2019.10.012
DO - 10.1016/j.jbiosc.2019.10.012
M3 - Article
C2 - 31837994
AN - SCOPUS:85076489346
VL - 129
SP - 624
EP - 631
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -