TY - JOUR
T1 - Enumeration of chemoorganotrophic carbonyl sulfide (COS)-degrading microorganisms by the most probable number method
AU - Kato, Hiromi
AU - Ogawa, Takahiro
AU - Ohta, Hiroyuki
AU - Katayama, Yoko
N1 - Funding Information:
This study was funded by Grants-in-Aid for Scientific Research from The Ministry of Education, Culture, Sport, and Technology of Japan (Grant No. 18310020 for Yoko Katayama; 17310018 for Hiroyuki Ohta). Field sampling was authorized by the Japanese Agency of Cultural Affairs, the Forestry Agency, the Ministry of the Environment, and other parties. We thank the staff at the Mt. Fuji Weather Station and staff at the non-profit organization “Valid Utilization of Mt. Fuji Weather Station” for their help with soil sampling.
Funding Information:
This study was funded by Grants-in-Aid for Scientific Research from The Ministry of Education, Culture, Sport, and Technology of Japan ?Grant No. 18310020 for Yoko Katayama; 17310018 for Hiroyuki Ohta). Field sampling was authorized by the Japanese Agency of Cultural Affairs, the Forestry Agency, the Ministry of the Environment, and other parties. We thank the staff at the Mt. Fuji Weather Station and staff at the non-profit organization “Valid Utilization of Mt. Fuji Weather Station” for their help with soil sampling.
Publisher Copyright:
© 2020, Japanese Society of Microbial Ecology. All rights reserved.
PY - 2020
Y1 - 2020
N2 - Carbonyl sulfide (COS) is the most abundant sulfur compound in the atmosphere, and, thus, is important in the global sulfur cycle. Soil is a major sink of atmospheric COS and the numerical distribution of soil microorganisms that degrade COS is indispensable for estimating the COS-degrading potential of soil. However, difficulties are associated with counting COS-degrading microorganisms using culture-dependent approaches, such as the most probable number (MPN) method, because of the chemical hydrolysis of COS by water. We herein developed a two-step MPN method for COS-degrading microorganisms: the first step for chemoorganotrophic growth that supported a sufficient number of cells for COS degradation in the second step. Our new MPN analysis of various environmental samples revealed that the cell density of COS-degrading microorganisms in forest soils ranged between 106 and 108 MPN (g dry soil)–1, which was markedly higher than those in volcanic deposit and water samples, and strongly correlated with the rate of COS degradation in environmental samples. Numerically dominant COS degraders that were isolated from the MPN-positive culture were related to bacteria in the orders Bacillales and Actinomycetales. The present results provide numerical evidence for the ubiquity of COS-degrading microbes in natural environments.
AB - Carbonyl sulfide (COS) is the most abundant sulfur compound in the atmosphere, and, thus, is important in the global sulfur cycle. Soil is a major sink of atmospheric COS and the numerical distribution of soil microorganisms that degrade COS is indispensable for estimating the COS-degrading potential of soil. However, difficulties are associated with counting COS-degrading microorganisms using culture-dependent approaches, such as the most probable number (MPN) method, because of the chemical hydrolysis of COS by water. We herein developed a two-step MPN method for COS-degrading microorganisms: the first step for chemoorganotrophic growth that supported a sufficient number of cells for COS degradation in the second step. Our new MPN analysis of various environmental samples revealed that the cell density of COS-degrading microorganisms in forest soils ranged between 106 and 108 MPN (g dry soil)–1, which was markedly higher than those in volcanic deposit and water samples, and strongly correlated with the rate of COS degradation in environmental samples. Numerically dominant COS degraders that were isolated from the MPN-positive culture were related to bacteria in the orders Bacillales and Actinomycetales. The present results provide numerical evidence for the ubiquity of COS-degrading microbes in natural environments.
KW - Carbonic anhydrase
KW - Carbonyl sulfide
KW - Most probable number method
KW - Soil microbes
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U2 - 10.1264/jsme2.ME19139
DO - 10.1264/jsme2.ME19139
M3 - Article
C2 - 32350165
AN - SCOPUS:85084170468
SN - 1342-6311
VL - 35
JO - Microbes and Environments
JF - Microbes and Environments
IS - 2
M1 - ME19139
ER -