Ensemble noise and current relaxation analysis of K+ current in single isolated salivary acinar cells from rat

Y. Maruyama, A. Nishiyama, T. Izumi, N. Hoshimiya, O. H. Petersen

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The K+ channel in rat parotid gland acinar cells were investigated by ensemble current noise analysis in single isolated cells employing the giga-seal whole cell current recording mode. Sets of 20-40 identical de- and hyperpolarization voltage steps were applied and the resultant current records were processed by computer to obtain the mean and the variance of the current. The time-course of the mean current could be fitted by the sum of two exponentials, suggesting a 3-state model. The simplest plausible hypothesis is a model with one open and two closed states. Assuming this model, the relationship between the variance (σ2) and the mean current (I) could be fitted by the function σ2/I=i-I/N. The estimated single channel i/V-relations were similar to those taken from single channel current recordings, and the size of the population of channels per cell (N) was 76±26 (n=12). The validity of the model was tested by a successful simulation of the time-course of the variance.

Original languageEnglish
Pages (from-to)69-72
Number of pages4
JournalPflügers Archiv European Journal of Physiology
Volume406
Issue number1
DOIs
Publication statusPublished - 1986 Jan 1

Keywords

  • Acinar cells
  • Current relaxation
  • Ensemble noise analysis
  • K channels
  • Patch-clamp whole cell recording

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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