## Abstract

The K^{+} channel in rat parotid gland acinar cells were investigated by ensemble current noise analysis in single isolated cells employing the giga-seal whole cell current recording mode. Sets of 20-40 identical de- and hyperpolarization voltage steps were applied and the resultant current records were processed by computer to obtain the mean and the variance of the current. The time-course of the mean current could be fitted by the sum of two exponentials, suggesting a 3-state model. The simplest plausible hypothesis is a model with one open and two closed states. Assuming this model, the relationship between the variance (σ_{2}) and the mean current (I) could be fitted by the function σ_{2}/I=i-I/N. The estimated single channel i/V-relations were similar to those taken from single channel current recordings, and the size of the population of channels per cell (N) was 76±26 (n=12). The validity of the model was tested by a successful simulation of the time-course of the variance.

Original language | English |
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Pages (from-to) | 69-72 |

Number of pages | 4 |

Journal | Pflügers Archiv European Journal of Physiology |

Volume | 406 |

Issue number | 1 |

DOIs | |

Publication status | Published - 1986 Jan 1 |

## Keywords

- Acinar cells
- Current relaxation
- Ensemble noise analysis
- K channels
- Patch-clamp whole cell recording

## ASJC Scopus subject areas

- Physiology
- Clinical Biochemistry
- Physiology (medical)

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