Enrichment and characterization of a bacterial mixture capable of utilizing C-mannosyl tryptophan as a carbon source

Tanim J. Hossain, Shino Manabe, Yukishige Ito, Toshiya Iida, Saori Kosono, Kenji Ueda, Akira Hosomi, Daishi Inoue, Tadashi Suzuki

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

C-Mannosylation, a protein-modification found in various eukaryotes, involves the attachment of a single mannose molecule to selected tryptophan residues of proteins. Since C-mannosyl tryptophan (CMW) was detected in human urine, it is generally thought that CMW is not catabolized inside our body and instead is excreted via the urine. This paper reports enrichment of a bacterial consortium from soil that degrades CMW. The bacteria grew in minimal medium supplemented with CMW as the carbon source. Interestingly, even after successive clonal picks of individual colonies, several species were still present in each colony as revealed by 16S rRNA gene sequence analysis, indicating that a single species may not be responsible for this activity. A next generation sequencing (NGS) analysis was therefore carried out in order to determine which bacteria were responsible for the catabolism of CMW. It was found that a species of Sphingomonadaceae family, but not others, increased with simultaneous decrease of CMW in the media, suggesting that this species is most likely the one that is actively involved in the degradation of CMW.

Original languageEnglish
Pages (from-to)165-176
Number of pages12
JournalGlycoconjugate Journal
Volume35
Issue number2
DOIs
Publication statusPublished - 2018 Apr 1
Externally publishedYes

Keywords

  • 16S rRNA gene sequence
  • Biodegradation
  • C-Mannosyl tryptophan
  • C-Mannosylation
  • Next generation sequencing
  • Sphingomonadaceae

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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