Enolase in the RNA degradosome plays a crucial role in the rapid decay of glucose transporter mRNA in the response to phosphosugar stress in Escherichia coli

Teppei Morita, Hiroshi Kawamoto, Taisei Mizota, Toshifumi Inada, Hiroji Aiba

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

The ptsG mRNA encoding the major glucose transporter is rapidly degraded in an RNase E-dependent manner in response to the accumulation of glucose 6-P or fructose 6-P when the glycolytic pathway is blocked at its early steps in Escherichia coli. RNase E, a major endonuclease, is associated with polynucleotide phosphorylase (PNPase), RhIB helicase and a glycolytic enzyme, enolase, which bind to its C-terminal scaffold region to form a multienzyme complex called the RNA degradosome. The role of enolase within the RNase E-based degradosome in RNA decay has been totally mysterious. In this article, we demonstrate that the removal of the scaffold region of RNase E suppresses the rapid degradation of ptsG mRNA in response to the metabolic stress without affecting the expression of ptsG mRNA under normal conditions. We also demonstrate that the depletion of enolase but not the disruption of pnp or rhIB eliminates the rapid degradation of ptsG mRNA. Taken together, we conclude that enolase within the degradosome plays a crucial role in the regulation of ptsG mRNA stability in response to a metabolic stress. This is the first instance in which a physiological role for enolase in the RNA degradosome has been demonstrated. In addition, we show that PNPase and RhIB within the degradosome cooperate to eliminate short degradation intermediates of ptsG mRNA.

Original languageEnglish
Pages (from-to)1063-1075
Number of pages13
JournalMolecular Microbiology
Volume54
Issue number4
DOIs
Publication statusPublished - 2004 Nov 1
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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