Enhancing effects of trichloroethylene and tetrachloroethylene on type I allergic responses in mice

Makoto Seo, Ryo Kobayashi, Tetsunori Okamura, Koji Ikeda, Masahiko Satoh, Naoki Inagaki, Hiroichi Nagai, Hisamitsu Nagase

Research output: Contribution to journalLetter

2 Citations (Scopus)

Abstract

Trichloroethylene (TCE) and tetrachloroethylene (perchloroethylene; PCE) are commonly identified as environmental contaminants of groundwater. Previously, we investigated the enhancing effects of TCE and PCE on antigen-induced histamine release and inflammatory mediator production in rat mast cells. In this study, to examine the potential effect of TCE and PCE on antigen-induced histamine release from mouse mast cells, mouse bone marrow-derived mast cells (BMMC) were sensitized with anti-dinitrophenol (DNP) monoclonal IgE antibody and then stimulated with DNP-BSA containing with TCE or PCE. Both TCE and PCE significantly enhanced antigen-induced histamine release from BMMC. Next we investigated the effects of TCE and PCE on the passive cutaneous anaphylaxis (PCA) reaction in vivo using ICR mice. TCE and PCE significantly enhanced the PCA reaction in a dose-dependent manner. In addition, we examined the enhancing effects of ingesting small amount of TCE and PCE in drinking water on antigen-stimulated allergic responses. After the ICR mice had ingested TCE or PCE in their drinking water for 2 or 4 weeks, we performed the PCA reaction. Both TCE and PCE ingestion enhanced the PCA reaction in a dose-dependent manner for 4 weeks. These results suggest that exposure to TCE and PCE leads to the augmentation of type I allergic responses in many species.

Original languageEnglish
Pages (from-to)439-445
Number of pages7
JournalJournal of Toxicological Sciences
Volume37
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1
Externally publishedYes

Keywords

  • BMMC
  • Histamine release
  • PCA reaction
  • Tetrachloroethylene
  • Trichloroethylene

ASJC Scopus subject areas

  • Toxicology

Fingerprint Dive into the research topics of 'Enhancing effects of trichloroethylene and tetrachloroethylene on type I allergic responses in mice'. Together they form a unique fingerprint.

  • Cite this