Enhancement of transgene expression in mammalian cell line by a Δ7-prostaglandin A1 analogue

Taichi Matsuda, Toshiyuki Takai, Masaki Hikida, Tatsuji Yasuda, Seizi Kurozumi, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

Abstract

We transfected a Chinese hamster ovary cell line, CHO-K1, with the gene of the Escherichia coli β-galactosidase (β-Gal) or the firefly luciferase (Luc) gene together with neoR gene, and isolated the stable transfectants, designated as CHO-Z neo and CHO-Z neo, respectively. When these cells were incubated for 24 to 72 h with 1.0 μg/ml TEI-3313, a Δ7-prostaglandin A1 analogue, the total and the specific activities of β-Gal and Luc were enhanced severalfold. It was confirmed by immunotitration experiments that the enhancement of β-Gal activity was due to an increase in the level of enzyme protein. Northern blot analysis revealed that this compound augmented the level of β-Gal mRNA. On the other hand, it did not significantly affect the expression of an endogenous gene such as that encoding lactate dehydrogenase or β-actin.

Original languageEnglish
Pages (from-to)281-283
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • luciferase
  • mammalian cell line
  • prostaglandin A derivative
  • stable gene expression
  • β-galactosidase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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