Enhancement of TNF-α Synthesis by Overexpression of Gαz in a Mast Cell Line

Rudolf A. Baumgartner, Noriyasu Hirasawa, Koichiro Ozawa, Fabian Gusovsky, Michael A. Beaven

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Ag stimulation of mast cells via the IgE receptor (Fc∈RI) elicits production and release of numerous cytokines. This activation of Fc∈RI initiates various tyrosine kinase-dependent signaling cascades, which ultimately result in the de novo synthesis of cytokines. To date, no heterotrimeric G proteins have been implicated in this process. Here we report that the a subunit of the heterotrimeric G protein, Gz, can regulate production of the cytokine, TNF-α. The α subunit was overexpressed in a cultured mast cell line (RBL-2H3) known to contain Gαz. In stimulated cells, overexpression of Gαz significantly enhanced the production of TNF-α. This effect of Gαz appeared to be restricted in that constitutive synthesis of the cytokine, TGF-β, and Ag-stimulation of the phosphoinositide-dependent secretory pathway were not significantly affected. Thus, Gaz, a heterotrimeric G protein, appeared to modulate the stimulatory pathways for induction of TNF-α synthesis in RBL-2H3 cells.

Original languageEnglish
Pages (from-to)1625-1629
Number of pages5
JournalJournal of Immunology
Volume157
Issue number4
Publication statusPublished - 1996 Aug 15
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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