Enhancement of lipid peroxidation and its amelioration by Vitamin E in a subject with mutations in the SBP2 gene

Yoshiro Saito, Mototada Shichiri, Takashi Hamajima, Noriko Ishida, Yuichiro Mita, Shohei Nakao, Yoshihisa Hagihara, Yasukazu Yoshida, Kazuhiko Takahashi, Etsuo Niki, Noriko Noguchi

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Selenocysteine (Sec) insertion sequence-binding protein 2 (SBP2) is essential for the biosynthesis of Sec-containing proteins, termed selenoproteins. Subjects with mutations in the SBP2 gene have decreased levels of several selenoproteins, resulting in a complex phenotype. Selenoproteins play a significant role in antioxidative defense, and defi ciencies in these proteins can lead to increased oxidative stress. However, lipid peroxidation and the effects of antioxidants in subjects with SBP2 gene mutations have not been studied. In the present study, we evaluated the lipid peroxidation products in the blood of a subject (the proband) with mutations in the SBP2 gene. We found that the proband had higher levels of free radical-mediated lipid peroxidation products, such as 7 β -hydroxycholesterol, than the control subjects. Treatment of the proband with Vitamin E ( α tocopherol acetate, 100 mg/day), a lipid-soluble antioxidant, for 2 years reduced lipid peroxidation product levels to those of control subjects. Withdrawal of Vitamin E treatment for 7 months resulted in an increase in lipid peroxidation products. Collectively, these results clearly indicate that free radical-mediated oxidative stress is increased in the subject with SBP2 gene mutations and that Vitamin E treatment effectively inhibits the generation of lipid peroxidation products.

Original languageEnglish
Pages (from-to)2172-2182
Number of pages11
JournalJournal of lipid research
Volume56
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1
Externally publishedYes

Keywords

  • Antioxidative defense
  • Cholesterol
  • Free radical
  • Oxidized lipids
  • Oxysterol
  • Selenocysteine insertion sequence-binding protein 2
  • Selenoprotein

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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