TY - JOUR
T1 - Enhancement of glucose-induced insulin secretion in transgenic mice overexpressing human VIP gene in pancreatic β cells
AU - Kato, Ichiro
AU - Suzuki, Yu
AU - Akabane, Atsuya
AU - Yonekura, Hideto
AU - Tanaka, Osamu
AU - Kondo, Hisatake
AU - Takasawa, Shin
AU - Yoshimoto, Takashi
AU - Okamoto, Hiroshi
PY - 1996
Y1 - 1996
N2 - Using transgenic mice technology, it has now become possible to test directly whether VIP and PHM-27 can enhance glucose-induced insulin secretion and reduce blood glucose in vivo. By microinjecting the entire human VIP gene ligated to the rat insulin II promoter, we have established a mouse model that overproduces VIP and PHM-27 in pancreatic P cells. VIP was secreted from transgenic islets in a glucose-dependent manner. Analyses of these VIP-transgenic mice indicated that the transgene efficiently enhances glucose-induced insulin secretion and significantly reduces blood glucose as compared with control mice. The transgene also ameliorated glucose intolerance of 70% depancreatized mice. The present results suggest that somatic cell gene therapy directed to diabetic islets by human VIP/PHM-27 gene introduction may provide a means to improve the secretory function of the diabetic islets.
AB - Using transgenic mice technology, it has now become possible to test directly whether VIP and PHM-27 can enhance glucose-induced insulin secretion and reduce blood glucose in vivo. By microinjecting the entire human VIP gene ligated to the rat insulin II promoter, we have established a mouse model that overproduces VIP and PHM-27 in pancreatic P cells. VIP was secreted from transgenic islets in a glucose-dependent manner. Analyses of these VIP-transgenic mice indicated that the transgene efficiently enhances glucose-induced insulin secretion and significantly reduces blood glucose as compared with control mice. The transgene also ameliorated glucose intolerance of 70% depancreatized mice. The present results suggest that somatic cell gene therapy directed to diabetic islets by human VIP/PHM-27 gene introduction may provide a means to improve the secretory function of the diabetic islets.
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U2 - 10.1111/j.1749-6632.1996.tb17486.x
DO - 10.1111/j.1749-6632.1996.tb17486.x
M3 - Article
C2 - 8993406
AN - SCOPUS:0030464705
VL - 805
SP - 232
EP - 243
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -
