Enhancement of glucose-induced insulin secretion in transgenic mice overexpressing human VIP gene in pancreatic β cells

Ichiro Kato, Yu Suzuki, Atsuya Akabane, Hideto Yonekura, Osamu Tanaka, Hisatake Kondo, Shin Takasawa, Takashi Yoshimoto, Hiroshi Okamoto

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Using transgenic mice technology, it has now become possible to test directly whether VIP and PHM-27 can enhance glucose-induced insulin secretion and reduce blood glucose in vivo. By microinjecting the entire human VIP gene ligated to the rat insulin II promoter, we have established a mouse model that overproduces VIP and PHM-27 in pancreatic P cells. VIP was secreted from transgenic islets in a glucose-dependent manner. Analyses of these VIP-transgenic mice indicated that the transgene efficiently enhances glucose-induced insulin secretion and significantly reduces blood glucose as compared with control mice. The transgene also ameliorated glucose intolerance of 70% depancreatized mice. The present results suggest that somatic cell gene therapy directed to diabetic islets by human VIP/PHM-27 gene introduction may provide a means to improve the secretory function of the diabetic islets.

Original languageEnglish
Pages (from-to)232-243
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume805
DOIs
Publication statusPublished - 1996

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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