TY - JOUR
T1 - Enhancement of expression of introduced β-galactosidase gene by 2-aminopurine in mammalian cell lines
AU - Tanigawa, Takahiro
AU - Hikida, Masaki
AU - Takai, Toshiyuki
AU - Ohmori, Hitoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (No. 04650877) from the Ministry of Education Science and Culture of Japan.
PY - 1993
Y1 - 1993
N2 - We transfected a mouse myeloma cell line, P3/NS1-Ag4-1 (NS-1), and a chinese hamster ovary cell line, CHO-K1, with the β-galactosidase (β-Gal) gene of Escherichia coli, and isolated stable transformants, designated as NS-1Z/gpt and CHO-Z/neo, respectively. When these cells were incubated with 5-10 mM 2-aminopurine (2-AP) for 12-24 h, the specific and the total activity of β-Gal was enhanced 2- to 4-fold and 1.5- to 2-fold, respectively. It was confirmed in immunotitration experiments that the enhancement of β-Gal activity was due to the increase of the enzyme protein. Northern blot analysis revealed that 2-AP augmented the expression of β-Gal mRNA. On the other hand, 2-AP did not significantly affect the expression of an endogenous gene like lactate dehydrogenase or β-actin.
AB - We transfected a mouse myeloma cell line, P3/NS1-Ag4-1 (NS-1), and a chinese hamster ovary cell line, CHO-K1, with the β-galactosidase (β-Gal) gene of Escherichia coli, and isolated stable transformants, designated as NS-1Z/gpt and CHO-Z/neo, respectively. When these cells were incubated with 5-10 mM 2-aminopurine (2-AP) for 12-24 h, the specific and the total activity of β-Gal was enhanced 2- to 4-fold and 1.5- to 2-fold, respectively. It was confirmed in immunotitration experiments that the enhancement of β-Gal activity was due to the increase of the enzyme protein. Northern blot analysis revealed that 2-AP augmented the expression of β-Gal mRNA. On the other hand, 2-AP did not significantly affect the expression of an endogenous gene like lactate dehydrogenase or β-actin.
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U2 - 10.1016/0922-338X(93)90072-G
DO - 10.1016/0922-338X(93)90072-G
M3 - Article
AN - SCOPUS:0027257766
VL - 76
SP - 145
EP - 147
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 2
ER -