Enhancement of DNA transfection efficiency by heat treatment of cultured mammalian cells

Toshiyuki Takai, Hitoshi Ohmori

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The expression of genes introduced into various mammalian cell lines was enhanced by raising the temperature of the cells to 42°C for a few hours after DNA transfection. This heat treatment resulted in an up to 10-fold increase in the frequency of the cells that transiently expressed a foreign gene such as that of β-galactosidase, whereas it had only a limited enhancing effect on the development of stable transformants. By immunotitration analysis, it was confirmed that the enhanced expression of β-galactosidase activity correlated well with the increase of the enzyme protein. This procedure may have an applicability for augmenting the frequency of transient gene expression in many cell types.

Original languageEnglish
Pages (from-to)161-165
Number of pages5
JournalBBA - Gene Structure and Expression
Volume1129
Issue number2
DOIs
Publication statusPublished - 1992 Jan 6
Externally publishedYes

Keywords

  • (Cultured mammalian cell)
  • DNA transfection
  • Heat shock
  • Transient expression

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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