Enhancement of 2- and 16α-estradiol hydroxylation in MCF-7 human breast cancer cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin

J. F. Gierthy, D. W. Lincoln, S. J. Kampcik, H. W. Dickerman, H. L. Bradlow, T. Niwa, G. E. Swaneck

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin exhibits antiestrogenic activity and induces cytochromes P-450 in estrogen-dependent MCF-7 human breast-cancer cells. To determine whether induction of 2- or 16α-hydroxylation of 17β-estradiol has a role in this antiestrogenic activity, MCF-7 cells which were exposed to this xenobiotic for 72 hrs were incubated with either [2-3H] or [16α-3H] 17β-estradiol and the extent of tritiated H2O formation, indicative of site-specific hydroxylation, was determined. 2,3,7,8-Tetrachlorodibenzo-p-dioxin-treated MCF-7 cultures showed an 8-fold increase in 2-hydroxylation and a 2-fold increase in 16α-hydroxylation. These results support the suggestion that increased hydroxylation of 17β-estradiol may have a role in the antiestrogenic activity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in MCF-7 cells.

Original languageEnglish
Pages (from-to)515-520
Number of pages6
JournalBiochemical and biophysical research communications
Volume157
Issue number2
DOIs
Publication statusPublished - 1988 Dec 15
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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