Enhanced UV sensitivity of yeast cells induced by overexpression of Mg2+-dependent protein phosphatase α (type 2C α)

Takayasu Kobayashi, Akira Yasui, Motoko Ohnishi, Shunsuke Kato, Yoji Sasahara, Kazuyuki Kusuda, Naoki Chida, Yuchio Yanagawa, Akira Hiraga, Shinri Tamura

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

The UV sensitivity of wild-type Saccharomyces cerevisiae cells was increased 2-fold when rat Mg2+-dependent protein phosphatase a (protein phosphatase type 2C α) was overexpressed in the cells. The overexpression of this enzyme rendered the rad 18 mutant (defective in postreplication repair) more UV-sensitive than was observed in the wild-type cells. However, this increase in UV sensitivity disappeared when the host cells had a rad 1 mutation (defective in excision repair). These results suggest that the Mg2+-dependent protein phosphatase overexpressed in the yeast cells inhibited their excision repair system.

Original languageEnglish
Pages (from-to)213-217
Number of pages5
JournalMutation Research - DNA Repair
Volume362
Issue number3
DOIs
Publication statusPublished - 1996 Apr 2

Keywords

  • Mg-dependent protein phosphatase
  • Overexpression
  • Saccharomyces cerevisiae
  • Ultraviolet sensitivity

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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