Enhanced uptake of giant DNA in cell-sized liposomes

Yuko Sato; Shin Ichiro M. Nomura; Kenichi Yoshikawa

doi:10.1016/j.cplett.2003.08.050

Enhanced uptake of giant DNA in cell-sized liposomes

Yuko Sato, Shin Ichiro M. Nomura, Kenichi Yoshikawa

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg²⁺ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg²⁺. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg²⁺.

Original language	English
Pages (from-to)	279-285
Number of pages	7
Journal	Chemical Physics Letters
Volume	380
Issue number	3-4
DOIs	https://doi.org/10.1016/j.cplett.2003.08.050
Publication status	Published - 2003 Oct 21
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy(all)
Physical and Theoretical Chemistry

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AU - Nomura, Shin Ichiro M.

AU - Yoshikawa, Kenichi

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Y1 - 2003/10/21

N2 - We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg2+ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg2+. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg2+.

AB - We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg2+ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg2+. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg2+.

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Enhanced uptake of giant DNA in cell-sized liposomes

Abstract

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