Enhanced saccharification of rice straw by overexpression of rice exo-glucanase

Mutsumi Nigorikawa, Aiko Watanabe, Kayoko Furukawa, Tomonori Sonoki, Yukihiro Ito

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background Efficient production of carbon-neutral biofuels is key to resolving global warming and exhaustion of fossil fuels. Cellulose, which is the most abundant biomass, is physically strong and biochemically stable, and these characteristics lead to difficulty of efficient saccharification of cellulosic compounds for production of fermentable glucose and other sugars. Results We transformed rice with overexpressing constructs of rice genes encoding each of three classes of cellulases. The exo-glucanase overexpressing plants showed various abnormalities in leaf such as division of leaf blade, crack on leaf surface, excess lacunae in midrib structure and necrotic colour change. The overexpressing plants also showed sterility. Noticeably, these plants showed enhanced saccharification of stems after maturation. These results indicate that overexpression of the exo-glucanase gene brought about various developmental defects associated with modification of cell wall and enhanced saccharification in rice. On the other hand, endo-glucanase-overexpressing plants could not be obtained, and overexpression of β-glucosidase brought about no effect on plant growth and development. Conclusions Our results indicate that genetic engineering of cellulosic biomass plants by overexpressing cellulase genes will be one of the approaches to confer enhanced saccharification ability for efficient production of cellulosic biofuels such as ethanol.

Original languageEnglish
Article number14
JournalRice
Volume5
Issue number1
DOIs
Publication statusPublished - 2012 Jan 1

Keywords

  • Bioethanol
  • Cell wall
  • Cellulase
  • Rice
  • Saccharification

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science
  • Plant Science

Fingerprint Dive into the research topics of 'Enhanced saccharification of rice straw by overexpression of rice exo-glucanase'. Together they form a unique fingerprint.

  • Cite this