Enhanced regeneration of critical bone defects using a biodegradable gelatin sponge and β-tricalcium phosphate with bone morphogenetic protein-2

Goichi Matsumoto, Yasushi Omi, Eiro Kubota, Satoru Ozono, Hideko Tsuzuki, Yukihiko Kinoshita, Masaya Yamamoto, Yasuhiko Tabata

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We examine the osteogenicity of a sponge biomaterial consisting of a biodegradable mixture of gelatin and β-tricalcium phosphate (βTCP) that bound bone morphogenetic protein 2 (BMP-2) in critical-sized bone defects in rats. Gelatin-βTCP sponges containing either phosphate buffered saline or incorporating BMP-2 are implanted into 5 mm diameter bone defects created in rat mandibles. We assess the defects biweekly for 8 weeks following implantation. There is significantly higher osteoinductive activity and significantly more Gla-osteocalcin content at bone-defect healing sites treated with gelatin-βTCP sponges incorporating BMP-2 than there is in those treated with sponges that did not contain BMP-2. Histologically, new bone that contains bone marrow and that is connected to the original bone almost entirely replaces the regenerated bone. These results show that biodegradable gelatin-βTCP incorporating BMP-2 is osteogenic enough to promote healing in large bone defects.

Original languageEnglish
Pages (from-to)327-342
Number of pages16
JournalJournal of Biomaterials Applications
Volume24
Issue number4
DOIs
Publication statusPublished - 2009 Nov 17
Externally publishedYes

Keywords

  • Bone morphogenetic protein
  • Controlled release
  • Gelatin-β-tricalcium phosphate (βTCP) sponge

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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