Enhanced osteogenesis by a biomimic pseudo-periosteum-involved tissue engineering Strategy

Xuetao Shi, Song Chen, Yihua Zhao, Chen Lai, Hongkai Wu

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    Elaborating a bone replacement using tissue-engineering strategies for bone repair seems to be a promising remedy. However, previous platforms are limited in constructing three-dimensional porous scaffolds and neglected the critical importance of periosteum (a pivotal source of osteogenic cells for bone regeneration). We report here an innovative method using the periosteum as a template to replicate its exquisite morphologies onto the surfaces of biomaterials. The precise topographic cues (grooved micropatterns) on the surface of collagen membrane inherited from the periosteum effectively directed cell alignment as the way of natural periosteum. Besides, we placed the stem-cell and endothelial-cell-laden collagen membrane (pseudo-periosteum) onto a three-dimensional porous scaffold. The pseudo-periosteum-covered scaffolds showed remarkable osteogenesis when compared with the pseudo-periosteum-free scaffolds, indicating the significant importance of pseudo-periosteum on bone regeneration. This study gives a novel concept for the construction of bone tissue engineering scaffold and may provide new insight for periosteum research. A newly developed collagen based pseudo-periosteum that simulates the motif of native perisoteum has a strong influence on spatially alignment and proliferation of stem cells. Importantly, the pseudo-periosteum-covered porous scaffold significantly promotes osteogenesis when compared with the pseudo-periosteum-free scaffold, indicating the important potential of periosteum in bone remolding and healing.

    Original languageEnglish
    Pages (from-to)1229-1235
    Number of pages7
    JournalAdvanced Healthcare Materials
    Volume2
    Issue number9
    DOIs
    Publication statusPublished - 2013 Sep

    Keywords

    • Co-culture
    • Micropatterns
    • Osteogenesis
    • Periosteum
    • Tissue engineering

    ASJC Scopus subject areas

    • Biomaterials
    • Biomedical Engineering
    • Pharmaceutical Science

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