Enhanced function of human immunodeficiency virus type 1 V3 loop for viral entry

Hong Ling, Hong Xi Gu, Dian Jun Li, Dao Hong Lin, Feng Min Zhang, Song Bin Fu, Toshio Hattori

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To elucidate the effect of the third variable (V3) loop in the early entry stage of human immunodeficiency virus type 1 (HIV-1). Methods: Three looped V3 peptides (V3-BH10, V3-ADA, V3-89.6) derived from three different HIV strains BH10 (X4), ADA (R5), 89.6(R5X4) were made. A non-loop control peptide V3-BH10/CA and its shorter forms V3-NNT-24 as well as V3-IRI12 were also synthesized. Three lentivirus-based luciferase reporter pseudoviruses bearing the envelop of HIV-1 HXB2, ADA or 89.6, respectively, were constructed. The effects of the peptides on the entry of various-tropic HIV-1 strains into target cells were examined. Results: (1) V3 peptides enhanced the entry of HIV-1 pseudoviruses HXB2, ADA, and 89.6 in a manner of virus-restriction. (2) Similar enhancement effect of linear peptide, V3-BH10/CA, was also found; its shorter form, V3-NNT24, which contains C-terminal of V3-BH10/CA, showed similar effect, while V2-IRI12, its shorter form containing only the central part did not show enhancement. Conclusion: The present study finds that HIV-1 V3 loop can enhance viral entry into target cells in a manner of strain-specificity for the first time. This finding will facilitate to understand the mechanism of HIV entry.

Original languageEnglish
Pages (from-to)275-278
Number of pages4
JournalChinese Journal of Microbiology and Immunology
Volume24
Issue number4
Publication statusPublished - 2004 Apr 1

Keywords

  • Human immunodeficiency virus type 1 (HIV-1)
  • Peptide
  • Target cells
  • V3 loop
  • Virus entry

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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