Enhanced expression of Rhizobium etli cbb

C. Talbi, C. Sánchez, A. Hidalgo-Garcia, E. M. González, C. Arrese-Igor, L. Girard, E. J. Bedmar, M. J. Delgado

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

To investigate the involvement of Rhizobium etli cbb3 oxidase in the response of Phaseolus vulgaris to drought, common bean plants were inoculated with the R. etli strain, CFNX713, overexpressing this oxidase in bacteroids (cbb3+) and subjected to drought conditions. The negative effect of drought on plant and nodule dryweight, nitrogen content, and nodule functionality was more pronounced in plants inoculated with the wild-type (WT) strain than in those inoculated with the cbb3+ strain. Regardless of the plant treatment, bacteroids produced by the cbb3+ strain showed higher respiratory capacity than those produced by the WT strain. Inoculation of plants with the cbb3+ strain alleviated the negative effect of a moderate drought on the respiratory capacity of bacteroids and the energy charge of the nodules. Expression of the FixP and FixO components of the cbb3 oxidase was higher in bacteroids of the cbb3+ strain than in those of the WT strain under all experimental conditions. The decline in sucrose synthase activity and the decrease in dicarboxylic acids provoked by moderate drought stress were more pronounced in nodules from plants inoculated with the WT strain than in those inoculated with the cbb3+ strain. Taken together, these results suggest that inoculation of plants with a R. etli strain having enhanced expression of cbb3 oxidase in bacteroids reduces the sensitivity of P. vulgaris-R. etli symbiosis to drought and can modulate carbon metabolism in nodules.

Original languageEnglish
Pages (from-to)5035-5043
Number of pages9
JournalJournal of experimental botany
Volume63
Issue number14
DOIs
Publication statusPublished - 2012 Sep
Externally publishedYes

Keywords

  • Bacteroidal respiration
  • Phaseolus vulgaris
  • Rhizobium etli
  • carbon metabolism
  • nodules

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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