TY - JOUR
T1 - Engineering systems for the generation of patterned co-cultures for controlling cell-cell interactions
AU - Kaji, Hirokazu
AU - Camci-Unal, Gulden
AU - Langer, Robert
AU - Khademhosseini, Ali
N1 - Funding Information:
This paper was partially supported by the National Institutes of Health ( EB008392 ; DE019024 ; HL092836 ; EB006365 ), the US Army Core of Engineers. HK acknowledges support from JSPS Postdoctoral Fellowships for Research Abroad.
PY - 2011/3
Y1 - 2011/3
N2 - Background: Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will benefit from methods of controlling cell-cell interactions with microscale resolution. Scope of the review: We discuss the use of microfabrication technologies for generating patterned co-cultures. In addition, we categorize patterned co-culture systems by cell type and discuss the implications of regulating cell-cell interactions in the resulting biological function of the tissues. Major conclusions: Patterned co-cultures are a useful tool for fabricating tissue engineered constructs and for studying cell-cell interactions in vitro, because they can be used to control the degree of homotypic and heterotypic cell-cell contact. In addition, this approach can be manipulated to elucidate important factors involved in cell-matrix interactions. General significance: Patterned co-culture strategies hold significant potential to develop biomimetic structures for tissue engineering. It is expected that they would create opportunities to develop artificial tissues in the future. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.
AB - Background: Inside the body, cells lie in direct contact or in close proximity to other cell types in a tightly controlled architecture that often regulates the resulting tissue function. Therefore, tissue engineering constructs that aim to reproduce the architecture and the geometry of tissues will benefit from methods of controlling cell-cell interactions with microscale resolution. Scope of the review: We discuss the use of microfabrication technologies for generating patterned co-cultures. In addition, we categorize patterned co-culture systems by cell type and discuss the implications of regulating cell-cell interactions in the resulting biological function of the tissues. Major conclusions: Patterned co-cultures are a useful tool for fabricating tissue engineered constructs and for studying cell-cell interactions in vitro, because they can be used to control the degree of homotypic and heterotypic cell-cell contact. In addition, this approach can be manipulated to elucidate important factors involved in cell-matrix interactions. General significance: Patterned co-culture strategies hold significant potential to develop biomimetic structures for tissue engineering. It is expected that they would create opportunities to develop artificial tissues in the future. This article is part of a Special Issue entitled Nanotechnologies - Emerging Applications in Biomedicine.
KW - Cell adhesion
KW - Cell-cell interaction
KW - Co-culture
KW - Microfabrication
KW - Micropatterning
KW - Tissue engineering
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U2 - 10.1016/j.bbagen.2010.07.002
DO - 10.1016/j.bbagen.2010.07.002
M3 - Review article
C2 - 20655984
AN - SCOPUS:79551583353
VL - 1810
SP - 239
EP - 250
JO - Biochimica et Biophysica Acta - General Subjects
JF - Biochimica et Biophysica Acta - General Subjects
SN - 0006-3002
IS - 3
ER -