Endoxyloglucan transferase is localized both in the cell plate and in the secretory pathway destined for the apoplast in tobacco cells

R. Yokoyama, K. Nishitani

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Intracellular trafficking of enzymes responsible for constructing and modifying the cell wall architecture in plants is mostly unknown. To examine their translocation pathways, we employed an endoxyloglucan transferase (EXGT), a key enzyme responsible for forming and rearranging the cellulose/xyloglucan network of the cell wall. We traced its intracellular localization in suspension-cultured cells of tobacco bright yellow-2 by means of green fluorescent protein-fusion gene procedures as well as by indirect immunofluorescence. During interphase the protein was extensively secreted into the apoplast via the endoplasmic reticulum-Golgi apparatus network, whereas during cytokinesis, the protein was exclusively located in the phragmoplast and eventually transported to the cell plate. These results clearly indicate commitment of EXGT protein to the construction of both the cell plate and the cell wall. This study also visualized the process of phragmoplast development at a level of vesicle translocation in the living cell.

Original languageEnglish
Pages (from-to)292-300
Number of pages9
JournalPlant and Cell Physiology
Volume42
Issue number3
DOIs
Publication statusPublished - 2001 Jan 1

Keywords

  • Cell plate
  • Cell wall
  • Endoxyloglucan transferase
  • Green fluorescence protein
  • Secretion
  • Tobacco

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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