Endothelial modulation of porcine coronary microcirculation perfused via immature collaterals

F. W. Sellke, Y. Kagaya, R. G. Johnson, T. Shafique, F. J. Schoen, W. Grossman, R. M. Weintraub

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Porcine hearts have relatively few native collateral vessels and lack the propensity to develop normal perfusion to the collateral-dependent myocardium. To examine microvascular responses in the collateral-dependent region, collateral vessels were stimulated in pigs by the Ameroid constrictor technique. After 4-7 wk, isolated microarterial vessels (90-170 μm ID) were studied in a pressurized (40 mm Hg), no-flow state. Microvessels from noninstrumented pigs were used as controls for vascular studies. Although myocardium in the collateral-dependent region showed minimal evidence of infarction, percent systolic shortening was reduced at rest and after pacing compared with myocardium in the normally perfused region. Relaxations to the receptor-mediated endothelium-dependent agents ADP and bradykinin were impaired in collateral-dependent coronary microvessels. Relaxations to the calcium ionophore A23187, which acts through a non-receptor-mediated mechanism, were similar in control and Ameroid microvessels. Relaxations to the endothelium-independent agent sodium nitroprusside were markedly enhanced in microvessels from the collateral-dependent region compared with microvessels from control hearts. In conclusion, receptor-mediated endothelium-dependent relaxation is impaired and endothelium-independent relaxation to sodium nitroprusside is enhanced in microvessels from myocardium perfused by immature collateral vessels.

Original languageEnglish
Pages (from-to)H1669-H1675
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume262
Issue number6 31-6
Publication statusPublished - 1992 Jan 1
Externally publishedYes

Keywords

  • adenosine 5'- diphosphate
  • bradykinin
  • calcium ionophore A23187
  • collateral vessels
  • coronary arteries
  • sodium nitroprusside

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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