Endothelial cells constituting blood-nerve barrier have highly specialized characteristics as barrier-forming cells

Yasuteru Sano, Fumitaka Shimizu, Hiroto Nakayama, Masaaki Abe, Toshihiko Maeda, Sumio Ohtsuki, Tetsuya Terasaki, Masuo Obinata, Masatsugu Ueda, Ri Ichi Takahashi, Takashi Kanda

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)


In autoimmune disorders of the peripheral nervous system (PNS) such as Guillain-Barré syndrome and chronic inflammatory demyelinating polyradiculoneuropathy, breakdown of the blood-nerve barrier (BNB) has been considered as a key step in the disease process. Hence, it is important to know the cellular property of peripheral nerve microvascular endothelial cells (PnMECs) constituting the bulk of BNB. Although many in vitro models of the blood-brain barrier (BBB) have been established, very few in vitro BNB models have been reported so far. We isolated PnMECs from transgenic rats harboring the temperature-sensitive SV40 large T-antigen gene (tsA58 rat) and investigated the properties of these "barrier-forming cells". Isolated PnMECs (TR-BNBs) showed high transendothelial electrical resistance and expressed tight junction components and various types of influx as well as efflux transporters that have been reported to function at BBB. Furthermore, we confirmed the in vivo expression of various BBB-forming endothelial cell markers in the endoneurium of a rat sciatic nerve. These results suggest that PnMECs constituting the bulk of BNB have a highly specialized characteristic resembling the endothelial cells forming BBB.

Original languageEnglish
Pages (from-to)139-147
Number of pages9
JournalCell structure and function
Issue number2
Publication statusPublished - 2007
Externally publishedYes


  • Blood-nerve barrier
  • Claudin-5
  • Peripheral nerve microvascular endothelial cells
  • Tight junction
  • Transporter

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology


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