TY - JOUR
T1 - Endothelial adhesion molecules in glomerular lesions
T2 - Association with their severity and diversity in lupus models
AU - Nakatani, Kimihiko
AU - Fujii, Hiroshi
AU - Hasegawa, Hitoshi
AU - Terada, Miho
AU - Arita, Norimasa
AU - Ito, Mitsuko R.
AU - Ono, Masao
AU - Takahashi, Satoru
AU - Saiga, Kan
AU - Yoshimoto, Shuhei
AU - Iwano, Masayuki
AU - Shiiki, Hideo
AU - Saito, Yoshihiko
AU - Nose, Masato
N1 - Funding Information:
This study was supported by the Grant-in Aid for Scientific Research Funds of the Ministry of Education, Science and Culture of Japan (#11307003 and #14370077 to M.N.), and with the help of the Integrated Center of Sciences (INCS), Ehime University. The authors are indebted to Ms. Hiromi Ohura for technical help with immunostaining and Dr. Herbert M. Schulman for critically reviewing the manuscript.
PY - 2004/4
Y1 - 2004/4
N2 - Background. To clarify whether vascular endothelial adhesion molecules in glomeruli might contribute to the severity and diversity of glomerular lesions in lupus nephritis, their expression in lupus models was analyzed. Methods. The expression levels of E- and P-selectin and vascular cell adhesion molecule-1 (VCAM-1) in glomeruli of different histopathologic grades of MRL/MpJ-lpr/lpr (MRL/lpr) lupus mice was studied using laser-capture microdissection of the glomeruli, followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis. The glomerular lesions in SCID mice injected with the 2B11.3 and 7B6. 8 clones, which are derived from an MRL/lpr mouse and induce endocapillary proliferative and wire loop type of glomerular lesions, respectively, were analyzed. To investigate the effect of a soluble form of E-selectin (sE-selectin) on the development of glomerular lesions, sE-selectin-producing L cells were prepared by transfection of the cDNA encoding sE-selectin and injected into SCID mice. Results. The glomeruli in MRL/lpr mice showed increased expression of these adhesion molecules, corresponding to the severity of the glomerular lesions. The endocapillary proliferative type lesions in SCID mice induced by the 2B11.3 clone showed significantly increased expression of the adhesion molecules, especially E-selectin and P-selectin, but the wire loop type lesion induced by the 7B6.8 clone expressed only VCAM-1. Formation of the endocapillary proliferative type lesions induced by the 2B11.3 clone was markedly prevented in association with elevation of the serum level of sE-selectin produced by the tansfected L cells. Conclusion. The severity and diversity of the histopathology of lupus nephritis are partially associated with the expression of vascular endothelial adhesion molecules in glomeruli.
AB - Background. To clarify whether vascular endothelial adhesion molecules in glomeruli might contribute to the severity and diversity of glomerular lesions in lupus nephritis, their expression in lupus models was analyzed. Methods. The expression levels of E- and P-selectin and vascular cell adhesion molecule-1 (VCAM-1) in glomeruli of different histopathologic grades of MRL/MpJ-lpr/lpr (MRL/lpr) lupus mice was studied using laser-capture microdissection of the glomeruli, followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis. The glomerular lesions in SCID mice injected with the 2B11.3 and 7B6. 8 clones, which are derived from an MRL/lpr mouse and induce endocapillary proliferative and wire loop type of glomerular lesions, respectively, were analyzed. To investigate the effect of a soluble form of E-selectin (sE-selectin) on the development of glomerular lesions, sE-selectin-producing L cells were prepared by transfection of the cDNA encoding sE-selectin and injected into SCID mice. Results. The glomeruli in MRL/lpr mice showed increased expression of these adhesion molecules, corresponding to the severity of the glomerular lesions. The endocapillary proliferative type lesions in SCID mice induced by the 2B11.3 clone showed significantly increased expression of the adhesion molecules, especially E-selectin and P-selectin, but the wire loop type lesion induced by the 7B6.8 clone expressed only VCAM-1. Formation of the endocapillary proliferative type lesions induced by the 2B11.3 clone was markedly prevented in association with elevation of the serum level of sE-selectin produced by the tansfected L cells. Conclusion. The severity and diversity of the histopathology of lupus nephritis are partially associated with the expression of vascular endothelial adhesion molecules in glomeruli.
KW - Histopathology
KW - Laser-capture microdissection
KW - Lupus nephritis
KW - MRL mice
KW - Soluble E-selectin
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U2 - 10.1111/j.1523-1755.2004.00537.x
DO - 10.1111/j.1523-1755.2004.00537.x
M3 - Article
C2 - 15086468
AN - SCOPUS:12144286302
VL - 65
SP - 1290
EP - 1300
JO - Kidney International
JF - Kidney International
SN - 0085-2538
IS - 4
ER -