Endothelial adhesion molecules in glomerular lesions: Association with their severity and diversity in lupus models

Kimihiko Nakatani, Hiroshi Fujii, Hitoshi Hasegawa, Miho Terada, Norimasa Arita, Mitsuko R. Ito, Masao Ono, Satoru Takahashi, Kan Saiga, Shuhei Yoshimoto, Masayuki Iwano, Hideo Shiiki, Yoshihiko Saito, Masato Nose

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Background. To clarify whether vascular endothelial adhesion molecules in glomeruli might contribute to the severity and diversity of glomerular lesions in lupus nephritis, their expression in lupus models was analyzed. Methods. The expression levels of E- and P-selectin and vascular cell adhesion molecule-1 (VCAM-1) in glomeruli of different histopathologic grades of MRL/MpJ-lpr/lpr (MRL/lpr) lupus mice was studied using laser-capture microdissection of the glomeruli, followed by reverse transcription-polymerase chain reaction (RT-PCR) analysis. The glomerular lesions in SCID mice injected with the 2B11.3 and 7B6. 8 clones, which are derived from an MRL/lpr mouse and induce endocapillary proliferative and wire loop type of glomerular lesions, respectively, were analyzed. To investigate the effect of a soluble form of E-selectin (sE-selectin) on the development of glomerular lesions, sE-selectin-producing L cells were prepared by transfection of the cDNA encoding sE-selectin and injected into SCID mice. Results. The glomeruli in MRL/lpr mice showed increased expression of these adhesion molecules, corresponding to the severity of the glomerular lesions. The endocapillary proliferative type lesions in SCID mice induced by the 2B11.3 clone showed significantly increased expression of the adhesion molecules, especially E-selectin and P-selectin, but the wire loop type lesion induced by the 7B6.8 clone expressed only VCAM-1. Formation of the endocapillary proliferative type lesions induced by the 2B11.3 clone was markedly prevented in association with elevation of the serum level of sE-selectin produced by the tansfected L cells. Conclusion. The severity and diversity of the histopathology of lupus nephritis are partially associated with the expression of vascular endothelial adhesion molecules in glomeruli.

Original languageEnglish
Pages (from-to)1290-1300
Number of pages11
JournalKidney international
Volume65
Issue number4
DOIs
Publication statusPublished - 2004 Apr
Externally publishedYes

Keywords

  • Histopathology
  • Laser-capture microdissection
  • Lupus nephritis
  • MRL mice
  • Soluble E-selectin

ASJC Scopus subject areas

  • Nephrology

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