Endogenous retroviral LTR DNA sequences as markers for individual human chromosomes

N. Nakamura, H. Sugino, K. Takahara, C. Jin, S. Fukushige, K. Matsubara

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The human genome carries multiple copies of sequences related to endogenous retroviral genomes that include long terminal repeat (LTR) sequences. We used the LTR of one such viral genome, called HERV-A, as a probe in Southern analysis to examine the distribution profiles of the hybridizing DNA in the genomes of twelve human x rodent hybrid cell lines carrying one or a few human chromosomes, and in the DNA samples prepared from six sorted, individual chromosomes. The HERV-A sequence was found to be widely distributed among different chromosomes and the Southern patterns for chromosomes 5, the X, and the Y, each obtained in duplicate from independently prepared cell lines or sorted chromosomes, were matched. Chromosome-specific Southern profiles can be used to monitor chromosomes in hybrid cells or to characterize chromosome aberrations, such as deletions.

Original languageEnglish
Pages (from-to)18-22
Number of pages5
JournalCytogenetics and Cell Genetics
Volume57
Issue number1
DOIs
Publication statusPublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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