Endo-xyloglucan transferase, a new class of transferase involved in cell wall construction

Kazuhiko Nishitani

    Research output: Contribution to journalArticlepeer-review

    59 Citations (Scopus)

    Abstract

    Cell shape in plants is constrained by cell walls, which are thick yet dynamic structures composed of crystalline cellulose microfibrils and matrix polymers. Xyloglucans are the principal component of the matrix polymers and bind tightly to the surface of cellulose microfibrils and thereby cross-link them to form an interwoven xyloglucan-cellulose network structure. Thus, cleavage and reconnection of the cross-links between xyloglucan molecules are required for the rearrangement of the cell wall architecture, the process essential for both cell wall expansion and the wall deposition occurring during cell growth and differentiation. Endoxyloglucan transferase (EXT) is a newly identified class of transferase that catalyzes molecular grafting between xyloglucan molecules. This enzyme catalyzes both endo-type splitting of a xyloglucan molecule and reconnection of a newly generated reducing terminus of the xyloglucan to the non-reducing terminus of another xyloglucan molecule, thereby mediating molecular grafting between xyloglucan cross-links in plant cell walls. Molecular cloning and sequencing of EXT-cDNAs derived from five different plant species including A. thaliana and V. angularis has revealed that the amino acid sequence of the mature protein is extensively conserved in the five different plant species, indicating that EXT protein is ubiquitous among higher plants. This structural study has also disclosed the presence of a group of xyloglucan related proteins (XRPs) with transferase activity in higher plants. Current data strongly suggest that these proteins are involved in a wide spectrum of physiological activities including cell wall expansion and deposition in growing cell walls.

    Original languageEnglish
    Pages (from-to)137-148
    Number of pages12
    JournalJournal of Plant Research
    Volume108
    Issue number1
    DOIs
    Publication statusPublished - 1995 Mar 1

    Keywords

    • Cell wall deposition
    • Endo-xyloglucan transferase (EXT)
    • Gene expression
    • Molecular grafting
    • Xyloglucan
    • Xyloglucan related protein (XRP)

    ASJC Scopus subject areas

    • Plant Science

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