Enantioselectivity of haloalkane dehalogenases and its modulation by surface loop engineering

Zbynek Prokop, Yukari Sato, Jan Brezovsky, Tomas Mozga, Radka Chaloupkova, Tana Koudelakova, Petr Jerabek, Veronika Stepankova, Ryo Natsume, Jan G.E. Van Leeuwen, Dick B. Janssen, Jan Florian, Yuji Nagata, Toshiya Senda, Jiri Damborsky

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Original languageEnglish
Pages (from-to)6111-6115
Number of pages5
JournalAngewandte Chemie - International Edition
Volume49
Issue number35
DOIs
Publication statusPublished - 2010 Aug 16

Keywords

  • Enantioselectivity
  • Enzymes
  • Haloalkane dehalogenases
  • Protein engineering
  • Thermodynamics

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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