Emission of ent-Kaurene, a diterpenoid hydrocarbon precursor for gibberellins, into the headspace from plants

Minoru Otsuka, Hiromichi Kenmoku, Mikihiro Ogawa, Kazunori Okada, Wataru Mitsuhashi, Takeshi Sassa, Yuji Kamiya, Tomonobu Toyomasu, Shinjiro Yamaguchi

    Research output: Contribution to journalArticlepeer-review

    35 Citations (Scopus)

    Abstract

    ent-Kaurene is a tetracyclic hydrocarbon precursor for gibberellins (GAs) in plants and fungi. To address whether fungal GA biosynthesis enzymes function in plants, we generated transgenic Arabidopsis plants overexpressing ent-kaurene synthase (GfCPS/KS) from a GA-producing fungus Gibberella fujikuroi. GfCPS/KS catalyzes a two-step reaction corresponding to ent-copalyl diphosphate synthase (CPS) and ent-kaurene synthase (KS) activities in plants. When GfCPS/KS was overexpressed and targeted to plastids, a range of GA-deficient phenotypes of the ga1-3 and ga2-1 mutants (defective in CPS and KS, respectively) were restored to wild type. Unexpectedly, the transgenic lines overproducing GfCPS/KS emitted the GA precursor ent-kaurene into the headspace besides its accumulation in the plant body. When co-cultivated with the ent-kaurene overproducers in a closed environment, the airborne ent-kaurene was able to fully complement the dwarf phenotype of ga1-3 and ga2-1 mutants, but not that of the ga3-1 mutant (defective in ent-kaurene oxidase). These results suggest that ent-kaurene may be efficiently metabolized into bioactive GAs in Arabidopsis when supplied as a volatile. We also provide evidence that ent-kaurene is released in the headspace of wild-type Chamaecyparis obtusa and Cryptomeria japonica plants, suggesting the occurrence of this hydrocarbon GA precursor as a volatile in nature.

    Original languageEnglish
    Pages (from-to)1129-1138
    Number of pages10
    JournalPlant and Cell Physiology
    Volume45
    Issue number9
    DOIs
    Publication statusPublished - 2004 Sep

    Keywords

    • Arabidopsis thaliana
    • Diterpene
    • Gibberella fujikuroi
    • Gibberellin
    • ent-Kaurene

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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