Emerging techniques for metagenome screening

Takefumi Shimoyama, Kazuya Watanabe

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Biotechnologists are interested in metagenomics as a tool to obtain novel, industrially valuable genes without the difficulties associated with the isolation and cultivation of microbes from the environment. Although environmental metagenomes harbor a huge diversity of microbial genes, only a small portion of them are accessible by conventional screening techniques. This greatly limits the utility of metagenomic approaches, and efforts to broaden accessible genes are therefore essential to make metagenomics more useful and attractive. In this chapter, two techniques that we have recently developed to access previously inaccessible genes in metagenomes are introduced, namely, substrate-induced gene-expression screening (SIGEX) and preferential annealing and removal of abundant metagenome fragments (PARM). SIGEX introduces a new concept in metagenome screening that relies on gene-expression activities rather than nucleotide sequences and enzyme activities, while PARM removes numerically abundant, redundant gene fragments from metagenomes in order to increase the possibility of cloning scarce gene fragments and construct a library of greater genetic diversity. We anticipate that metagenomics will be further advanced by introducing these techniques as standard protocols.

Original languageEnglish
Title of host publicationMetagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies
PublisherNova Science Publishers, Inc.
Pages321-336
Number of pages16
ISBN (Print)9781616686826
Publication statusPublished - 2011 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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