Objectives: To evaluate the presence of qnr genes among enterobacterial isolates carrying extended-spectrum beta-lactamases (ESBLs). Methods: Screening for the qnrA, qnrB and qnrS genes was carried out by PCR amplification with specific primers in 163 nonduplicate, clinically relevant ESBL-producing enterobacterial isolates collected between 2000 and 2005. ESBLs from all qnr-positive isolates were identified by PCR amplification and DNA sequencing. Pulsed-field gel electrophoresis was used to investigate the clonality of qnr-carrying isolates. Results: Seven Citrobacter freundii isolates, each from a different individual, carried qnrB. Among them, 4 had qnrB17, 2 had qnrB10 and 1 had qnrB12. None of the isolates carried qnrA and qnrS. Among the qnrB17-carrying isolates, 3 possessed blaTEM-3 alone and 1had both blaCTX-M-15 and blaOXA-1. The isolates with qnrB10 possessed blaTEM-3. The qnrB12-carrying isolate had bla CTX-M-15, blaOXA-1and blaTEM-1. Conclusions: The prevalence of qnr among enterobacterial clinical isolates carrying ESBLs between 2000 and 2005 was low (4.3%). Only qnrB was found and only in C. freundii isolates dating since 2002. qnrA and qnrS were not detected.
|Number of pages||2|
|Journal||Problems of Infectious and Parasitic Diseases|
|Publication status||Published - 2008|
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology (medical)
- Infectious Diseases