TY - JOUR
T1 - Emergence of H274Y oseltamivir-resistant A(H1N1) influenza viruses in Japan during the 2008-2009 season
AU - Baranovich, Tatiana
AU - Saito, Reiko
AU - Suzuki, Yasushi
AU - Zaraket, Hassan
AU - Dapat, Clyde
AU - Caperig-Dapat, Isolde
AU - Oguma, Taeko
AU - Shabana, Iman Ibrahim
AU - Saito, Takehiko
AU - Suzuki, Hiroshi
N1 - Funding Information:
Japan grants-in-aid for scientific research from Special Research of Health and Labor Sciences Research Grants ( Ministry of Education, Culture, Sports, Science and Technology, Japan ) and the Acute Respiratory Infections Panel, US-Japan Cooperative Medical Science Program ( Department of Health and Human Services and Department of State, United States , and Ministry of Foreign Affairs , Ministry of Health, Labor, and Welfare , and Ministry of Education, Culture, Sports, Science and Technology, Japan ).
PY - 2010/1
Y1 - 2010/1
N2 - Background: A substantial increase in oseltamivir-resistant A(H1N1) influenza viruses was reported in Europe in late 2007. Objectives: To monitor the antiviral susceptibility profile of human A(H1N1) influenza viruses in Japan during the 2007-2008 and 2008-2009 seasons. Study design: Viruses were obtained from respiratory samples of patients with influenza collected in Japan between December 2007 and April 2008 (n = 1046) and between December 2008 and April 2009 (n = 1789). Oseltamivir resistance was determined by an H274Y-specific real-time PCR cycling probe assay and a neuraminidase inhibition assay. Amantadine resistance was assessed by sequencing the M2 gene. Sequencing of the hemagglutinin and NA genes was performed to infer phylogenetic relationships between different strains. Results: Three of 687 (0.4%) A(H1N1) viruses from the 2007-2008 season and 745 of 745 (100%) viruses from the 2008-2009 season carried the NA-H274Y substitution and demonstrated a >300-fold reduction in oseltamivir susceptibility. All oseltamivir-resistant viruses from the 2008-2009 season possessed an A193T substitution in the receptor-binding domain of the hemagglutinin. Amantadine resistance was detected in 431 of 687 (62.7%) and 0 of 745 (0.0%) of the A(H1N1) viruses from the 2007-2008 and 2008-2009 seasons, respectively. Conclusions: A dramatic surge in oseltamivir-resistant A(H1N1) viruses possessing the NA-H274Y substitution was detected in Japan during the 2008-2009 season. The emergence of oseltamivir-resistant viruses was facilitated by mutations in the viral genome. Intensified surveillance, including phenotypic assays and sequencing of the hemagglutinin, neuraminidase, and M2 gene would allow monitoring of the spread and evolution of drug-resistant influenza virus variants.
AB - Background: A substantial increase in oseltamivir-resistant A(H1N1) influenza viruses was reported in Europe in late 2007. Objectives: To monitor the antiviral susceptibility profile of human A(H1N1) influenza viruses in Japan during the 2007-2008 and 2008-2009 seasons. Study design: Viruses were obtained from respiratory samples of patients with influenza collected in Japan between December 2007 and April 2008 (n = 1046) and between December 2008 and April 2009 (n = 1789). Oseltamivir resistance was determined by an H274Y-specific real-time PCR cycling probe assay and a neuraminidase inhibition assay. Amantadine resistance was assessed by sequencing the M2 gene. Sequencing of the hemagglutinin and NA genes was performed to infer phylogenetic relationships between different strains. Results: Three of 687 (0.4%) A(H1N1) viruses from the 2007-2008 season and 745 of 745 (100%) viruses from the 2008-2009 season carried the NA-H274Y substitution and demonstrated a >300-fold reduction in oseltamivir susceptibility. All oseltamivir-resistant viruses from the 2008-2009 season possessed an A193T substitution in the receptor-binding domain of the hemagglutinin. Amantadine resistance was detected in 431 of 687 (62.7%) and 0 of 745 (0.0%) of the A(H1N1) viruses from the 2007-2008 and 2008-2009 seasons, respectively. Conclusions: A dramatic surge in oseltamivir-resistant A(H1N1) viruses possessing the NA-H274Y substitution was detected in Japan during the 2008-2009 season. The emergence of oseltamivir-resistant viruses was facilitated by mutations in the viral genome. Intensified surveillance, including phenotypic assays and sequencing of the hemagglutinin, neuraminidase, and M2 gene would allow monitoring of the spread and evolution of drug-resistant influenza virus variants.
KW - A(H1N1)
KW - Amantadine
KW - Influenza
KW - Oseltamivir
KW - Resistance
KW - Zanamivir
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U2 - 10.1016/j.jcv.2009.11.003
DO - 10.1016/j.jcv.2009.11.003
M3 - Article
C2 - 19962344
AN - SCOPUS:72049131299
VL - 47
SP - 23
EP - 28
JO - Journal of Clinical Virology
JF - Journal of Clinical Virology
SN - 1386-6532
IS - 1
ER -
