Embryogenic staging of fugu, Takifugu rubripes, and expression profiles of aldh1a2, aldh1a3 and cyp26a1

Susumu Uji, Tadahide Kurokawa, Hisashi Hashimoto, Tohru Kasuya, Tohru Suzuki

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Fugu (Takifugu rubripes) has contributed as an ideal model organism for understanding the structure and evolution of vertebrate genomes, but also has potential as a good model organism for developmental biology because of the availability of the genome information. However, there is no comprehensive report describing the developmental stages, which is fundamental data for developmental biology. Here we describe a series of stages of the embryonic development of fugu during the first 8days after fertilization, i.e. from fertilization to hatching. We define seven periods of embryogenesis - the zygote, cleavage, blastula, gastrula, segmentation, pharyngula, and hatching periods. Stages subdividing these periods are defined based on morphological characteristics. In addition, as a model experiment of gene expression analysis using this staging series, we performed in situ hybridization of aldh1a2, aldh1a3 and cyp26a1 that play regulatory roles in retinoic acid (RA) metabolism essential for embryogenesis. This report provides fundamental information on fugu embryogenesis, which is anticipated to facilitate the use of fugu as a model organism for developmental studies.

Original languageEnglish
Pages (from-to)715-725
Number of pages11
JournalDevelopment Growth and Differentiation
Volume53
Issue number5
DOIs
Publication statusPublished - 2011 Jun

Keywords

  • Embryogenesis
  • Fugu
  • aldh1a2
  • aldh1a3
  • cyp26a1

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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