Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase

Mika K. Kaneko, Shinji Yamada, Shunsuke Itai, Yao Wen Chang, Takuro Nakamura, Miyuki Yanaka, Hiroyuki Harada, Hiroyoshi Suzuki, Yukinari Kato

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.

Original languageEnglish
Pages (from-to)101-103
Number of pages3
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume38
Issue number3
DOIs
Publication statusPublished - 2019 Jun

Keywords

  • TERT
  • epitope mapping
  • monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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