Eliminating transmissibility of bovine spongiform encephalopathy by dry-heat treatment

Yuichi Matsuura, Yukiko Ishikawa, Yuichi Murayama, Takashi Yokoyama, Robert A. Somerville, Tetsuyuki Kitamoto, Shirou Mohri

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Bovine spongiform encephalopathy (BSE) prion is more resistant to heat inactivation compared to other prions, but the effect of heat inactivation has been reported to differ depending on the BSE-contaminated tissue state or heating type. We aimed to evaluate the secure level of inactivation of original BSE transmissibility by dry-heating. Cattle tissues affected with BSE were subjected to dry-heat treatment for 20 min at various temperatures ranging from 150 to 1000 °C. To assess the inactivation effect, we conducted protein misfolding cyclic amplification (PMCA) and follicular dendritic cell (FDC) assays in transgenic mice expressing bovine prion protein genes. Under dry-heating at 600 °C or higher, BSE cattle tissues lost their transmissibility in transgenic mice. In contrast, transmissibility was detected in the cattle tissues treated at temperatures of 400 °C or lower through the FDC assay combined with PMCA. In this study, we confirmed that transmissibility was eliminated in BSE-affected cattle tissues by dry-heating at 600 °C or higher.

Original languageEnglish
Pages (from-to)136-142
Number of pages7
JournalJournal of General Virology
Issue number1
Publication statusPublished - 2020


  • Bovine spongiform encephalopathy
  • Dry-heating
  • Prion
  • Transgenic mice
  • Transmissibility

ASJC Scopus subject areas

  • Virology


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