TY - JOUR
T1 - Eliminating transmissibility of bovine spongiform encephalopathy by dry-heat treatment
AU - Matsuura, Yuichi
AU - Ishikawa, Yukiko
AU - Murayama, Yuichi
AU - Yokoyama, Takashi
AU - Somerville, Robert A.
AU - Kitamoto, Tetsuyuki
AU - Mohri, Shirou
N1 - Funding Information:
This study was supported in part by a Grant-in-Aid from the BSE and other Prion Disease Control Project of the Ministry of Agriculture, Forestry and Fisheries of Japan; a part by a Grant-in-Aid from the Food Safety Commission, Cabinet Office, Government of Japan; a part by Grants-in-Aid from The Research Committee of Prion Disease and Slow Virus Infection, the Ministry of Health, Labour and Welfare of Japan.
Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - Bovine spongiform encephalopathy (BSE) prion is more resistant to heat inactivation compared to other prions, but the effect of heat inactivation has been reported to differ depending on the BSE-contaminated tissue state or heating type. We aimed to evaluate the secure level of inactivation of original BSE transmissibility by dry-heating. Cattle tissues affected with BSE were subjected to dry-heat treatment for 20 min at various temperatures ranging from 150 to 1000 °C. To assess the inactivation effect, we conducted protein misfolding cyclic amplification (PMCA) and follicular dendritic cell (FDC) assays in transgenic mice expressing bovine prion protein genes. Under dry-heating at 600 °C or higher, BSE cattle tissues lost their transmissibility in transgenic mice. In contrast, transmissibility was detected in the cattle tissues treated at temperatures of 400 °C or lower through the FDC assay combined with PMCA. In this study, we confirmed that transmissibility was eliminated in BSE-affected cattle tissues by dry-heating at 600 °C or higher.
AB - Bovine spongiform encephalopathy (BSE) prion is more resistant to heat inactivation compared to other prions, but the effect of heat inactivation has been reported to differ depending on the BSE-contaminated tissue state or heating type. We aimed to evaluate the secure level of inactivation of original BSE transmissibility by dry-heating. Cattle tissues affected with BSE were subjected to dry-heat treatment for 20 min at various temperatures ranging from 150 to 1000 °C. To assess the inactivation effect, we conducted protein misfolding cyclic amplification (PMCA) and follicular dendritic cell (FDC) assays in transgenic mice expressing bovine prion protein genes. Under dry-heating at 600 °C or higher, BSE cattle tissues lost their transmissibility in transgenic mice. In contrast, transmissibility was detected in the cattle tissues treated at temperatures of 400 °C or lower through the FDC assay combined with PMCA. In this study, we confirmed that transmissibility was eliminated in BSE-affected cattle tissues by dry-heating at 600 °C or higher.
KW - Bovine spongiform encephalopathy
KW - Dry-heating
KW - Prion
KW - Transgenic mice
KW - Transmissibility
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U2 - 10.1099/JGV.0.001335
DO - 10.1099/JGV.0.001335
M3 - Article
C2 - 31718739
AN - SCOPUS:85078816568
VL - 101
SP - 136
EP - 142
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 1
ER -