TY - JOUR
T1 - Elevation of the thromboxane A2/prostacyclin ratio in urine of diabetic mice analyzed by gas chromatography/selected ion monitoring
AU - Hishinuma, Takanori
AU - Koseki, Yuko
AU - Murai, Yuriko
AU - Kotake, J.
AU - Ishii, Fumio
AU - Suzuki, Ken Ichi
AU - Mizugaki, Michinao
N1 - Funding Information:
A part of this study was supported by Grants-in-Aid for Scientific Research on Priority Areas and Grants-in-Aid for Developmental Scientific Research from The Ministry of Education, Science and Culture Japan. We are grateful to Dr. Y. Kurosaki, Upjohn Pharmaceuticals Limited, for the gift of [ 2 H 4 ]2,3-dinor-6-keto-PGF 1α .
PY - 1998/2
Y1 - 1998/2
N2 - Levels of the stable urinary metabolites of thromboxane B2 and prostacyclin, 11-dehydro-thromboxane B2 (11-dehydro-TXB2), and 2,3-dinor- 6-keto-prostaglandin F(1α) (2,3-dinor-6-keto-PGF(1α)) were measured in diabetic mice to elucidate the relation between the thromboxane A2/prostacyclin (TX/PGI) balance and pathological states of diabetes mellitus. 11-dehydro-TXB2 and 2,3-dinor-6-keto-PGF(1α) were converted to 1- methyl ester-propylamide-9,12,15-tris-dimethylisopropylsilyl (DMIPS) ether derivative and 1-methyl ester-6-methoxime-9,12,15-tris-DMIPS ether derivative respectively, and applied to a gas chromatography/selected ion monitoring (GC/SIM). The urinary levels of 11-dehydro-TXB2 and 2,3-dinor-6-keto- PGF(1α) in streptozotocin (STZ)-treated mice showed a tendency to be higher and lower than those of non-treated mice, respectively. As a result, the TX/PGI ratios of the STZ diabetic mice were higher than those of normal mice. Furthermore, the ratios of db/db mutant mice, rodent models of genetic diabetes, were increased with the progress of diabetes and showed positive correlations with the levels of blood glucose, hemoglobin A(1c), and triglyceride, and negative correlation with insulin levels. These observations indicate that the TX/PGI ratio reflects the pathological condition of diabetes and partly explain the hypercoagulation in this disease.
AB - Levels of the stable urinary metabolites of thromboxane B2 and prostacyclin, 11-dehydro-thromboxane B2 (11-dehydro-TXB2), and 2,3-dinor- 6-keto-prostaglandin F(1α) (2,3-dinor-6-keto-PGF(1α)) were measured in diabetic mice to elucidate the relation between the thromboxane A2/prostacyclin (TX/PGI) balance and pathological states of diabetes mellitus. 11-dehydro-TXB2 and 2,3-dinor-6-keto-PGF(1α) were converted to 1- methyl ester-propylamide-9,12,15-tris-dimethylisopropylsilyl (DMIPS) ether derivative and 1-methyl ester-6-methoxime-9,12,15-tris-DMIPS ether derivative respectively, and applied to a gas chromatography/selected ion monitoring (GC/SIM). The urinary levels of 11-dehydro-TXB2 and 2,3-dinor-6-keto- PGF(1α) in streptozotocin (STZ)-treated mice showed a tendency to be higher and lower than those of non-treated mice, respectively. As a result, the TX/PGI ratios of the STZ diabetic mice were higher than those of normal mice. Furthermore, the ratios of db/db mutant mice, rodent models of genetic diabetes, were increased with the progress of diabetes and showed positive correlations with the levels of blood glucose, hemoglobin A(1c), and triglyceride, and negative correlation with insulin levels. These observations indicate that the TX/PGI ratio reflects the pathological condition of diabetes and partly explain the hypercoagulation in this disease.
KW - Diabetes mellitus
KW - GC
KW - MS
KW - Prostacyclin
KW - Prostaglandin
KW - Thromboxane
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U2 - 10.1016/S0090-6980(98)00012-4
DO - 10.1016/S0090-6980(98)00012-4
M3 - Article
AN - SCOPUS:0031998999
VL - 55
SP - 83
EP - 93
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
SN - 0090-6980
IS - 2-3
ER -
