Elevation of the thromboxane A2/prostacyclin ratio in urine of diabetic mice analyzed by gas chromatography/selected ion monitoring

Takanori Hishinuma, Yuko Koseki, Yuriko Murai, J. Kotake, Fumio Ishii, Ken Ichi Suzuki, Michinao Mizugaki

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13 Citations (Scopus)

Abstract

Levels of the stable urinary metabolites of thromboxane B2 and prostacyclin, 11-dehydro-thromboxane B2 (11-dehydro-TXB2), and 2,3-dinor- 6-keto-prostaglandin F(1α) (2,3-dinor-6-keto-PGF(1α)) were measured in diabetic mice to elucidate the relation between the thromboxane A2/prostacyclin (TX/PGI) balance and pathological states of diabetes mellitus. 11-dehydro-TXB2 and 2,3-dinor-6-keto-PGF(1α) were converted to 1- methyl ester-propylamide-9,12,15-tris-dimethylisopropylsilyl (DMIPS) ether derivative and 1-methyl ester-6-methoxime-9,12,15-tris-DMIPS ether derivative respectively, and applied to a gas chromatography/selected ion monitoring (GC/SIM). The urinary levels of 11-dehydro-TXB2 and 2,3-dinor-6-keto- PGF(1α) in streptozotocin (STZ)-treated mice showed a tendency to be higher and lower than those of non-treated mice, respectively. As a result, the TX/PGI ratios of the STZ diabetic mice were higher than those of normal mice. Furthermore, the ratios of db/db mutant mice, rodent models of genetic diabetes, were increased with the progress of diabetes and showed positive correlations with the levels of blood glucose, hemoglobin A(1c), and triglyceride, and negative correlation with insulin levels. These observations indicate that the TX/PGI ratio reflects the pathological condition of diabetes and partly explain the hypercoagulation in this disease.

Original languageEnglish
Pages (from-to)83-93
Number of pages11
JournalProstaglandins and Other Lipid Mediators
Volume55
Issue number2-3
DOIs
Publication statusPublished - 1998 Feb
Externally publishedYes

Keywords

  • Diabetes mellitus
  • GC
  • MS
  • Prostacyclin
  • Prostaglandin
  • Thromboxane

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Pharmacology
  • Cell Biology

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