Elevation of oxidized DJ-1 in the brain and erythrocytes of Parkinson disease model animals

Yoko Ogawa Akazawa, Yoshiro Saito, Takao Hamakubo, Yoshinori Masuo, Yasukazu Yoshida, Keiko Nishio, Mototada Shichiri, Tomohiro Miyasaka, Hiroko Iwanari, Yasuhiro Mochizuki, Tatsuhiko Kodama, Noriko Noguchi, Etsuo Niki

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

DJ-1, the causative gene of a familial form of Parkinson's disease (PD), has been reported undergo oxidation preferentially at the 106th cysteine residue (Cys-106) under oxidative stress. Recently, it has been found that the levels of oxidized DJ-1 in erythrocytes of unmedicated PD patients are markedly higher than those in medicated PD patients and healthy subjects. In the present study, we examined the changes in oxidized DJ-1 levels in the brain and erythrocytes of PD animal models using specific antibodies against Cys-106-oxidized DJ-1. Treatment with PD model compounds such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and 6-hydroxydopamine significantly elevated the levels of oxidized DJ-1 in erythrocytes. Immunohistochemical analysis also revealed that the number of oxidized DJ-1 antibody-positive cells in the substantia nigra of MPTP-treated mouse increased in a dose-dependent manner. These results suggest that the oxidative modification of DJ-1 in the brain and erythrocytes is involved in the pathogenesis of PD in animal models.

Original languageEnglish
Pages (from-to)201-205
Number of pages5
JournalNeuroscience Letters
Volume483
Issue number3
DOIs
Publication statusPublished - 2010 Oct 1
Externally publishedYes

Keywords

  • Antibody
  • Biomarker
  • DJ-1
  • Enzyme-linked immunosorbent assay
  • Erythrocytes
  • Oxidative stress
  • Parkinson's disease

ASJC Scopus subject areas

  • Neuroscience(all)

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