TY - JOUR
T1 - Elevated substance P content in induced sputum from patients with asthma and patients with chronic bronchitis
AU - Tomaki, Masafumi
AU - Ichinose, Masakazu
AU - Miura, Motohiko
AU - Hirayama, Yoshitaka
AU - Yamauchi, Hideyuki
AU - Nakajima, Natsuko
AU - Shirato, Kunio
PY - 1995/3
Y1 - 1995/3
N2 - In experimental studies, tachykinins, especially substance P (SP), cause many of the pathophysiological features of neurogenic inflammation. It is unclear whether these peptides are involved in human airway inflammation in diseases such as asthma and chronic bronchitis. To elucidate the relation between neurogenic inflammation and airway inflammatory diseases, we examined the SP concentration in sputum after hypertonic saline inhalation challenge in patients with asthma, patients with chronic bronchitis, and normal volunteers. SP concentration was measured by radioimmunoassay. The sputum SP concentration was significantly higher in patients with asthma (mean ± SEM, 17.7 ± 2.4 fmol/ml; p < 0.01) and patients with chronic bronchitis (25.6 ± 5.5 fmol/ml; p < 0.01) than in normal volunteers (1.1 ± 0.4 fmol/ml). In patients with asthma, the SP concentration was significantly related to the eosinophil cell count in induced sputum. In all subjects, the SP concentration in induced sputum correlated with FEV1/FVC. These data suggest that neurogenic inflammation may be involved in the airway inflammatory process and subsequent airway narrowing not only in asthma but also in chronic bronchitis.
AB - In experimental studies, tachykinins, especially substance P (SP), cause many of the pathophysiological features of neurogenic inflammation. It is unclear whether these peptides are involved in human airway inflammation in diseases such as asthma and chronic bronchitis. To elucidate the relation between neurogenic inflammation and airway inflammatory diseases, we examined the SP concentration in sputum after hypertonic saline inhalation challenge in patients with asthma, patients with chronic bronchitis, and normal volunteers. SP concentration was measured by radioimmunoassay. The sputum SP concentration was significantly higher in patients with asthma (mean ± SEM, 17.7 ± 2.4 fmol/ml; p < 0.01) and patients with chronic bronchitis (25.6 ± 5.5 fmol/ml; p < 0.01) than in normal volunteers (1.1 ± 0.4 fmol/ml). In patients with asthma, the SP concentration was significantly related to the eosinophil cell count in induced sputum. In all subjects, the SP concentration in induced sputum correlated with FEV1/FVC. These data suggest that neurogenic inflammation may be involved in the airway inflammatory process and subsequent airway narrowing not only in asthma but also in chronic bronchitis.
UR - http://www.scopus.com/inward/record.url?scp=0028931315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028931315&partnerID=8YFLogxK
U2 - 10.1164/ajrccm.151.3.7533601
DO - 10.1164/ajrccm.151.3.7533601
M3 - Article
C2 - 7533601
AN - SCOPUS:0028931315
VL - 151
SP - 613
EP - 617
JO - American Journal of Respiratory and Critical Care Medicine
JF - American Journal of Respiratory and Critical Care Medicine
SN - 1073-449X
IS - 3 I
ER -