Elemental analysis of cellular samples by in-air micro-PIXE

K. Ishii; A. Sugimoto; A. Tanaka; T. Satoh; S. Matsuyama; H. Yamazaki; C. Akama; T. Amartivan; H. Endoh; Y. Oishi; H. Yuki; S. Sugihara; M. Satoh; T. Kamiya; T. Sakai; K. Arakawa; M. Saidoh; S. Oikawa

doi:10.1016/S0168-583X(01)00623-1

Elemental analysis of cellular samples by in-air micro-PIXE

K. Ishii, A. Sugimoto, A. Tanaka, T. Satoh, S. Matsuyama, H. Yamazaki, C. Akama, T. Amartivan, H. Endoh, Y. Oishi, H. Yuki, S. Sugihara, M. Satoh, T. Kamiya, T. Sakai, K. Arakawa, M. Saidoh, S. Oikawa

ENG - Quantum Science and Energy Engineering

Research output: Contribution to journal › Conference article › peer-review

35 Citations (Scopus)

Abstract

We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.

Original language	English
Pages (from-to)	448-453
Number of pages	6
Journal	Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume	181
Issue number	1-4
DOIs	https://doi.org/10.1016/S0168-583X(01)00623-1
Publication status	Published - 2001 Jul
Event	7th International Conference on Nuclear Microprobe Technology (ICNMTA-2000) - Bordeaux, France Duration: 2000 Sep 10 → 2000 Sep 15

Keywords

In-air PIXE
Micro-PIXE analysis
Quantitative micro-PIXE analysis
Target preparation of cellular samples

ASJC Scopus subject areas

Nuclear and High Energy Physics
Instrumentation

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10.1016/S0168-583X(01)00623-1

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Ishii, K., Sugimoto, A., Tanaka, A., Satoh, T., Matsuyama, S., Yamazaki, H., Akama, C., Amartivan, T., Endoh, H., Oishi, Y., Yuki, H., Sugihara, S., Satoh, M., Kamiya, T., Sakai, T., Arakawa, K., Saidoh, M., & Oikawa, S. (2001). Elemental analysis of cellular samples by in-air micro-PIXE. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, 181(1-4), 448-453. https://doi.org/10.1016/S0168-583X(01)00623-1

Elemental analysis of cellular samples by in-air micro-PIXE. / Ishii, K.; Sugimoto, A.; Tanaka, A.; Satoh, T.; Matsuyama, S.; Yamazaki, H.; Akama, C.; Amartivan, T.; Endoh, H.; Oishi, Y.; Yuki, H.; Sugihara, S.; Satoh, M.; Kamiya, T.; Sakai, T.; Arakawa, K.; Saidoh, M.; Oikawa, S.

In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, Vol. 181, No. 1-4, 07.2001, p. 448-453.

Research output: Contribution to journal › Conference article › peer-review

Ishii, K, Sugimoto, A, Tanaka, A, Satoh, T, Matsuyama, S, Yamazaki, H, Akama, C, Amartivan, T, Endoh, H, Oishi, Y, Yuki, H, Sugihara, S, Satoh, M, Kamiya, T, Sakai, T, Arakawa, K, Saidoh, M & Oikawa, S 2001, 'Elemental analysis of cellular samples by in-air micro-PIXE', Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms, vol. 181, no. 1-4, pp. 448-453. https://doi.org/10.1016/S0168-583X(01)00623-1

Ishii, K. ; Sugimoto, A. ; Tanaka, A. ; Satoh, T. ; Matsuyama, S. ; Yamazaki, H. ; Akama, C. ; Amartivan, T. ; Endoh, H. ; Oishi, Y. ; Yuki, H. ; Sugihara, S. ; Satoh, M. ; Kamiya, T. ; Sakai, T. ; Arakawa, K. ; Saidoh, M. ; Oikawa, S. / Elemental analysis of cellular samples by in-air micro-PIXE. In: Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms. 2001 ; Vol. 181, No. 1-4. pp. 448-453.

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AU - Ishii, K.

AU - Sugimoto, A.

AU - Tanaka, A.

AU - Satoh, T.

AU - Matsuyama, S.

AU - Yamazaki, H.

AU - Akama, C.

AU - Amartivan, T.

AU - Endoh, H.

AU - Oishi, Y.

AU - Yuki, H.

AU - Sugihara, S.

AU - Satoh, M.

AU - Kamiya, T.

AU - Sakai, T.

AU - Arakawa, K.

AU - Saidoh, M.

AU - Oikawa, S.

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N2 - We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.

AB - We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.

KW - In-air PIXE

KW - Micro-PIXE analysis

KW - Quantitative micro-PIXE analysis

KW - Target preparation of cellular samples

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AN - SCOPUS:17144443113

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SP - 448

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JO - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

JF - Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms

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T2 - 7th International Conference on Nuclear Microprobe Technology (ICNMTA-2000)

Y2 - 10 September 2000 through 15 September 2000

ER -

Elemental analysis of cellular samples by in-air micro-PIXE

Abstract

Keywords

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