Elemental analysis of cellular samples by in-air micro-PIXE

K. Ishii, A. Sugimoto, A. Tanaka, T. Satoh, S. Matsuyama, H. Yamazaki, C. Akama, T. Amartivan, H. Endoh, Y. Oishi, H. Yuki, S. Sugihara, M. Satoh, T. Kamiya, T. Sakai, K. Arakawa, M. Saidoh, S. Oikawa

Research output: Contribution to journalConference articlepeer-review

33 Citations (Scopus)

Abstract

We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.

Original languageEnglish
Pages (from-to)448-453
Number of pages6
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume181
Issue number1-4
DOIs
Publication statusPublished - 2001 Jul 1
Event7th International Conference on Nuclear Microprobe Technology (ICNMTA-2000) - Bordeaux, France
Duration: 2000 Sep 102000 Sep 15

Keywords

  • In-air PIXE
  • Micro-PIXE analysis
  • Quantitative micro-PIXE analysis
  • Target preparation of cellular samples

ASJC Scopus subject areas

  • Nuclear and High Energy Physics
  • Instrumentation

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