Abstract
We developed a micro-PIXE system for the analysis of frozen cellular samples at atmospheric pressure under helium gas cooling. We analyzed bovine aortic endothelial (BAE) cells previously cultured in a medium containing bromodeoxyuridine (BrdU). It is known that these cells take BrdU into their nuclei at the S phase of their metabolic cycle. We examined the Br uptake for incubation times of 6, 12 and 24 h, respectively. The Br maps showed an accumulation in the nuclei and the Br concentration was found strongly dependent on the incubation time. We have demonstrated that in-air micro-PIXE analysis is a relevant technique for examining the molecular uptake in cells.
Original language | English |
---|---|
Pages (from-to) | 448-453 |
Number of pages | 6 |
Journal | Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms |
Volume | 181 |
Issue number | 1-4 |
DOIs | |
Publication status | Published - 2001 Jul |
Event | 7th International Conference on Nuclear Microprobe Technology (ICNMTA-2000) - Bordeaux, France Duration: 2000 Sep 10 → 2000 Sep 15 |
Keywords
- In-air PIXE
- Micro-PIXE analysis
- Quantitative micro-PIXE analysis
- Target preparation of cellular samples
ASJC Scopus subject areas
- Nuclear and High Energy Physics
- Instrumentation