Electrostatic and hydrodynamic separation of DNA fragments in capillary tubes

Nobuhiko Iki, Yongseong Kim, Edward S. Yeung

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Two separation mechanisms are demonstrated for DNA fragments in free solution inside narrow capillary tubes. No sieving medium or buffer additive is needed. In the electromigration mode, a likely model is that dissociated silanol groups on the capillary walls create an excess of positive charges in the diffuse layer near the surface. These provide electrostatic shielding of the negatively charged DNA fragments to lower their electrophoretic mobility against electroosmotic flow. Small fragments can access the diffuse layer more readily because of lower steric hindrance and larger diffusion coefficients and are therefore eluted earlier than the large fragments. In the hydrodynamic mode, the Poisseuille flow profile causes molecules near the surface to move slower. So, the smaller DNA fragments are eluted later than the larger fragments.

Original languageEnglish
Pages (from-to)4321-4325
Number of pages5
JournalAnalytical Chemistry
Volume68
Issue number24
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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