Electroporation of adherent cells by direct lamination of hydrogel-based microelectrode substrates

Daisuke Takahashi, Masato Sasaki, Kuniaki Nagamine, Keiichi Torimitsu, Matsuhiko Nishizawa

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide. Electropolymerization of poly(3,4- ethylenedioxythiophene) (PEDOT) anchors the Au electrodes to the hydrogels. The resulting soft, molecularly permeable hydrogel-based microelectrodes were effective for the electroporation of adherent cells by direct lamination. The electroporation-assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human induced pluripotent stem (iPS) cells.

Original languageEnglish
Pages (from-to)444-446
Number of pages3
JournalChemistry Letters
Volume43
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

ASJC Scopus subject areas

  • Chemistry(all)

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