Electroporation into cultured mammalian embryos

Tadashi Nomura, Masanori Takahashi, Noriko Osumi

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Over the last century, mammalian embryos have been used extensively as a common animal model to investigate fundamental questions in the field of developmental biology. More recently, the establishment of transgenic and gene-targeting systems in laboratory mice has enabled researchers to unveil the genetic mechanisms under lying complex developmental processes (Mak, 2007). However, our understanding of cell--cell interactions and their molecular basis in the early stages of mammalian embryogenesis is still very fragmentary. One of the major problems is the difficulty of precise manipulation and limited accessibility to mammalian embryos via uterus wall. Unfortunately, existing tissue and organotypic culture systems per se do not fully recapitulate three-dimensional, dynamic processes of organogenesis observed in vivo. Although transgenic animal technology and virus-mediated gene delivery are useful to manipulate gene expression, these techniques take much time and financial costs, which limit their use. Whole-embryo mammalian culture system was established by New and colleague in the 1970s, and was modified thereafter by several researchers (reviewed by New, 1971, 1978; Sturm and Tam, 1993; Hogan et al., 1994; Eto and Osumi-Yamashita, 1995; Tam, 1998). At first, the whole-embryo culture system was used in the field of teratology, and then applied in a variety of developmental biology fields, because this system well maintains the embryonic growth and morphogenesis that are comparable to those in utero, and dramatically improves accessibility to the early fetal stages. Furthermore, in combination with gene-delivery techniques such as electroporation, gene expression can be manipulated in a tissue- and region-specific manner (Osumi and Inoue, 2001; Takahashi et al., 2002). Here we introduce this combinatorial proce dure applying of the electroporation technique to whole-embryo culture system. We then illustrate the use of this approach in the developmental neurobiology by present ing our findings on molecular mechanisms controlling stem cell maintenance and neuronal migration in the embryonic cortex. Finally, we will discuss future directions and a potential widespread value of this method in developmental biology.

Original languageEnglish
Title of host publicationElectroporation and Sonoporation in Developmental Biology
PublisherSpringer Japan
Pages129-141
Number of pages13
ISBN (Print)9784431094265
DOIs
Publication statusPublished - 2009 Dec 1

ASJC Scopus subject areas

  • Materials Science(all)
  • Chemistry(all)

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