Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Kuniaki Nagamine; Tomoyuki Yasukawa; Hitoshi Shiku; Masahiro Koide; Tomosato Itayama; Tomokazu Matsue

Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Masahiro Koide, Tomosato Itayama, Tomokazu Matsue

Research output: Contribution to conference › Paper › peer-review

Abstract

We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.

Original language	English
Pages	645-647
Number of pages	3
Publication status	Published - 2006 Jan 1
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

Electrochemistry
Electrophoresis
Microfluidic device
Single cell

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device. / Nagamine, Kuniaki; Yasukawa, Tomoyuki; Shiku, Hitoshi et al.

2006. 645-647 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

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title = "Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device",

abstract = "We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.",

keywords = "Electrochemistry, Electrophoresis, Microfluidic device, Single cell",

author = "Kuniaki Nagamine and Tomoyuki Yasukawa and Hitoshi Shiku and Masahiro Koide and Tomosato Itayama and Tomokazu Matsue",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "645--647",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

AU - Nagamine, Kuniaki

AU - Yasukawa, Tomoyuki

AU - Shiku, Hitoshi

AU - Koide, Masahiro

AU - Itayama, Tomosato

AU - Matsue, Tomokazu

PY - 2006/1/1

Y1 - 2006/1/1

N2 - We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.

AB - We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.

KW - Electrochemistry

KW - Electrophoresis

KW - Microfluidic device

KW - Single cell

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M3 - Paper

AN - SCOPUS:84902517347

SP - 645

EP - 647

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Abstract

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Keywords

ASJC Scopus subject areas

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