Electrophoretic karyotype and gene assignment to chromosomes of aspergillus oryzae

Katsuhiko Kitamoto, Kazuhiro Kimura, Katsuya Gomi, Chieko Kumagai

Research output: Contribution to journalArticle

Abstract

An electrophoretic karyotype of Aspergillus oryzae was obtained by transverse altering-field ele-ctrophoresis. Seven chromosomal bands were found. With Schizosaccharomyces pombe chromosomes as size standards, we estimated the sizes of the chromosomes to be 7.0, 5.2, 5.0, 4.5, 4.0, 3.7, and 2.8 megabase pairs (Mbp). The chromosomal DNA bands were identified with use of 13 cloned genes including ribosomal DNA. The intensity of ethidium staining and the results of Southern blotting with 100 random clones isolated from A. oryzae suggested that the smallest band migrated as doublet and that the total genome size was approximately 35 Mbp.

Original languageEnglish
Pages (from-to)1467-1470
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume58
Issue number8
DOIs
Publication statusPublished - 1994 Jan 1
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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