Electrooxidation of NADH with Co(phen)33+ by NADH dehydrogenase purified from Bacillus stearothermophilus

Tomokazu Matsue, Hiroshi Yamada, Hsien Chang Chang, Isamu Uchida

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The NADH dehydrogenase purified from Bacillus stearothermophilus catalyzes effectively the electrochemical oxidation of NADH mediated by Co(phen)3+3. Stopped-flow measurements indicate that the rate constant for the oxidation of the reduced enzyme by Co(phen)3+3 is 5 × 106 M-1 s-1 and that for the oxidation of NADH by the oxidized enzyme > 3 × 107 M-1 s-1. The voltammograms, showing catalytic prepeaks, were simulated using the above rate constants.

Original languageEnglish
Pages (from-to)347-354
Number of pages8
JournalBioelectrochemistry and Bioenergetics
Volume24
Issue number3
DOIs
Publication statusPublished - 1990 Dec

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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